FastQCFastQC Report
Sat 18 Mar 2023
SRR4895406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843254
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC1849250.0143.52981
ACTCACC1849500.0143.510422
CTCACCG626000.0142.88143
TCACCGA178500.0142.38364
CTCACCT681400.0142.073963
CTCACCA554500.0142.049353
TCACCGT233050.0142.019794
TCACCTA207750.0141.70974
TCACCGG216950.0141.574394
TCACCTG223650.0141.228454
TCACCAG187600.0141.003484
CACCTAG70850.0140.440285
CACCGTA70550.0140.016955
CACCGAT66800.0139.793525
TCACCAT207800.0139.666034
CACCGGT81950.0138.637765
ACCTTAG28050.0138.606756
CACCTGA64050.0138.601335
ACCGGAT23200.0138.410936
TCACCTT259250.0137.526184
Produced by FastQC (version 0.11.5)