FastQCFastQC Report
Sat 18 Mar 2023
SRR4895406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843254
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102420.5556477837563353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG67720.36739375040010763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC49030.26599698142524036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT496950.0138.703551
GGTATCA500550.0137.396458
GTGGTAT502150.0137.001666
TGGTATC502800.0136.724327
GTATCAA504950.0136.641229
AGCAGTG504900.0136.163042
CAGTGGT506550.0135.783224
AGTGGTA507950.0135.423165
GCAGTGG512800.0133.995163
ATCAACG501750.028.03921310-14
CAACGCA504850.027.99254210-14
TCAACGC503300.027.98719610-14
AGAGTAC505700.027.82050315-19
CAGAGTA507500.027.70480515-19
TACATGG504500.027.67665720-24
GTACATG507050.027.5687120-24
AACGCAG514450.027.52616310-14
CGCAGAG511700.027.46896415-19
AGTACAT511000.027.40915120-24
GCAGAGT511850.027.39620615-19
Produced by FastQC (version 0.11.5)