FastQCFastQC Report
Sat 18 Mar 2023
SRR4895405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094628
Sequences flagged as poor quality0
Sequence length150
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1096300.0143.772142
TTCACGC1097400.0143.628021
CACGCAT404200.0143.603563
ACGCATT149350.0143.368744
ACGCATG133100.0143.292224
CACGCAG366250.0143.287753
CACGCAA327900.0143.183013
ACGCAGT137600.0143.105934
CGCATAG41450.0142.953255
ACGCATA122050.0142.93364
ACGCAAT120350.0142.798954
CGCATGA36850.0142.627765
CGCATTA44450.0142.375695
ACGCAGG126850.0142.065664
ACGCAGA103100.0141.900454
CGCATAT42650.0141.800895
CGCAAGT40900.0141.7075
ACGCAAG113800.0141.59134
CGCAGTA44500.0141.568545
CGCATGT51000.0141.454355
Produced by FastQC (version 0.11.5)