FastQCFastQC Report
Sat 18 Mar 2023
SRR4895405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094628
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56480.5159743766832202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT29920.2733348681013093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT28380.2592661616549184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC23280.21267499095583156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG16340.14927445671040757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG15630.14278823490720136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC12310.11245829633446248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT11680.10670291642457527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11250.10277464124798562No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT11090.1013129574613476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187300.0139.301458
AAGCAGT188400.0139.067671
GTGGTAT188600.0138.608476
TGGTATC189850.0137.354557
CAGTGGT190450.0137.337664
GTATCAA190500.0137.188259
AGTGGTA191750.0136.481645
AGCAGTG193150.0135.349492
GCAGTGG196950.0132.76853
ATCAACG187550.028.3452210-14
AGAGTAC186650.028.2661515-19
TCAACGC188700.028.19536810-14
TACATGG186050.028.14988320-24
CAGAGTA187800.028.13139515-19
GTACATG188900.027.8547720-24
GAGTACA187200.027.70772620-24
AGTACAT191000.027.661620-24
GCAGAGT191900.027.52285815-19
CAACGCA194250.027.47873710-14
ACATGGG191950.027.23962420-24
Produced by FastQC (version 0.11.5)