FastQCFastQC Report
Sat 18 Mar 2023
SRR4895404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888731
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA1899000.0143.212252
GTGGTAA544300.0142.062063
GTGGTAG633450.0141.822393
GTGGTAT740150.0141.327973
TGGTAAG183000.0139.665594
TGGTATA207050.0139.611914
TGGTGGT1948450.0139.577641
TGGTAGA172600.0139.154544
TGGTATG256050.0138.847634
TGGTAGG227050.0137.587924
TGGTAAT215450.0137.376714
GGTATAG74400.0136.058145
TGGTAGT247900.0135.687064
GGTATTA81000.0135.54925
TGGTAAA159550.0135.464664
GGTAGTA71050.0135.385
GGTATGA68600.0134.337745
GGTAGAA54150.0133.090715
GGTAGGA63100.0133.039735
GGTATGG93850.0132.409365
Produced by FastQC (version 0.11.5)