FastQCFastQC Report
Sat 18 Mar 2023
SRR4895404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888731
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88730.4697863274336049No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG75560.4000569694678597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC51780.2741523276739779No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT42590.22549531934404635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC32170.17032600195581055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA26900.14242366964909242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21240.11245645886047298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA19660.10409105372866756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19170.10149671922576586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT473550.0138.492251
GGTATCA475700.0137.639288
GTGGTAT476050.0137.492726
GTATCAA479350.0137.026819
TGGTATC477500.0136.999827
AGTGGTA482100.0135.767295
AGCAGTG482550.0135.43182
CAGTGGT484000.0135.264074
GCAGTGG487850.0133.989993
ATCAACG476500.028.11322810-14
CAACGCA479050.028.07479710-14
TCAACGC478700.028.04117810-14
AGAGTAC479200.027.91876815-19
CAGAGTA479500.027.87727515-19
TACATGG477100.027.8007920-24
GTACATG478350.027.76727720-24
CGCAGAG482900.027.6690715-19
AGTACAT483100.027.61349120-24
GCAGAGT483400.027.56895815-19
AACGCAG487750.027.56221610-14
Produced by FastQC (version 0.11.5)