FastQCFastQC Report
Sat 18 Mar 2023
SRR4895403_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895403_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447130
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA1449600.0143.746752
GCTTCAG506900.0143.654223
GCTTCAA419800.0143.411973
TGGCTTC1453600.0143.35121
GCTTCAT524450.0143.26753
CTTCAGA140350.0142.353554
CTTCATG180450.0142.040044
CTTCAGT198050.0141.741184
CTTCAAG139600.0141.57114
CTTCAGG174600.0141.356024
CTTCATA156900.0140.69124
CTTCAAT159100.0140.374854
TCAGGAT18900.0137.519126
TTCAGGA48950.0137.376335
CTTCATT199650.0136.71064
TTCATGA51800.0136.489555
TTCAGAT55200.0136.169275
TTCATAG57450.0136.099815
TTCATGG72200.0136.017525
CTTCAAA132250.0135.992324
Produced by FastQC (version 0.11.5)