FastQCFastQC Report
Sat 18 Mar 2023
SRR4895403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447130
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92390.6384360769246716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA70600.48786218238858986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA59320.40991479687381227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT29550.20419727322355283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22840.15782963520899987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19200.13267640087621707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19100.13198537795498677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA18530.12804654730397408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG18440.12742462667486681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA18030.12459143269782258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC17790.12293297768686987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC15470.106901245914327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT15110.1044135633978979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA15020.10379164276879065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACTCTTTCGAGGCCCTG14880.10282421067906822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG14760.10199498317359186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCAAATTACCCACT14690.10151126712873067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT636800.0140.32341
GTGGTAT644150.0138.527456
AGTGGTA645250.0138.280145
TGGTATC644300.0138.27177
GGTATCA644500.0138.239978
CAGTGGT646900.0137.88294
GTATCAA648000.0137.871069
AGCAGTG648000.0137.54252
GCAGTGG653100.0136.452713
ATCAACG645950.028.21009810-14
CAACGCA649200.028.14650210-14
TCAACGC648400.028.1190510-14
AGAGTAC647050.027.99078415-19
CAGAGTA647550.027.95360815-19
AACGCAG654000.027.95313510-14
TACATGG641550.027.92217320-24
CGCAGAG652650.027.86092815-19
GCAGAGT649250.027.81387915-19
GTACATG646300.027.79939720-24
ACATGGG642850.027.72234720-24
Produced by FastQC (version 0.11.5)