FastQCFastQC Report
Sat 18 Mar 2023
SRR4895402_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895402_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495430
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA1500600.0143.496771
GAACAAT557400.0143.41453
GGAACAA1501550.0143.405982
GAACAAG523350.0142.950213
AACAATG186600.0141.063374
GAACAAA429800.0140.226683
AACAAGG187100.0140.147664
AACAAGT195300.0139.535064
ACAATGG69500.0139.126835
ACAATGA51900.0137.753175
ACAATAG60250.0137.662325
ACAATTG79300.0137.549535
CAAGGTA23100.0137.138816
ACAAGTA59350.0136.474495
ACAAGTG68600.0136.229225
AACAAGA154700.0136.083914
ACAATGT69600.0136.030475
ACAAGGT70450.0136.024375
CAATAGG22650.0135.095356
AACAATT219050.0134.431094
Produced by FastQC (version 0.11.5)