FastQCFastQC Report
Sat 18 Mar 2023
SRR4895402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495430
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61580.4117879138441786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG59460.3976113893662692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC36380.24327450967280315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21660.14484128310920605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT18510.12377710758778412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT385900.0138.586151
GTGGTAT385600.0138.133826
GGTATCA385950.0138.008568
TGGTATC387400.0137.473427
GTATCAA390550.0136.88089
CAGTGGT392300.0135.866424
AGTGGTA392900.0135.585655
AGCAGTG393200.0135.500522
GCAGTGG397500.0133.962273
CAACGCA387300.028.22678610-14
TCAACGC388250.028.08353810-14
ATCAACG387400.028.08196810-14
AGAGTAC387200.028.08159815-19
CAGAGTA389100.027.92597215-19
TACATGG385450.027.91396320-24
GTACATG386400.027.90496420-24
CGCAGAG390550.027.78910815-19
AGTACAT391300.027.70640620-24
GCAGAGT392250.027.65031615-19
AACGCAG397250.027.49803410-14
Produced by FastQC (version 0.11.5)