FastQCFastQC Report
Sat 18 Mar 2023
SRR4895401_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895401_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738075
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG741200.0143.384451
GAAGAGA743200.0142.998582
AAGAGAT277400.0141.686453
AAGAGAG257750.0141.426543
AAGAGAA217700.0139.895453
AGAGATG110300.0128.787374
AGAGAGT101500.0127.681544
AGAGATA87000.0127.196834
AGAGAAG85100.0125.383484
GAGATGG39000.0125.350725
GAGATTG42100.0124.500455
AGAGAAT87300.0124.285564
AGAGATT112750.0124.009334
GAGATTA33250.0123.425495
AGAGAGG105650.0123.1431664
GAGAGAT29550.0122.7989655
GAGATAG31150.0122.269835
GAGAATG30750.0122.2213445
GAGAGTA31750.0121.546585
GAGAATA26400.0121.36065
Produced by FastQC (version 0.11.5)