FastQCFastQC Report
Sat 18 Mar 2023
SRR4895401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738075
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37610.5095688107577143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA17900.24252277885038784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT16250.22016732716864817No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC12920.17504996104731904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12880.1745080107035193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG10420.1411780645598347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8540.11570639840124648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT183900.0139.809391
GGTATCA183850.0139.612438
GTGGTAT184050.0139.460726
GTATCAA185700.0138.531759
TGGTATC185250.0138.47967
CAGTGGT186600.0137.632084
AGCAGTG186600.0137.516332
AGTGGTA186950.0137.258885
GCAGTGG188050.0136.455983
ATCAACG184050.028.39287210-14
TCAACGC185100.028.27070810-14
AGAGTAC184350.028.22950215-19
CAGAGTA185050.028.15384315-19
CAACGCA187500.027.96260310-14
TACATGG184050.027.95473520-24
GTACATG184650.027.93408620-24
AGTACAT186550.027.84255420-24
GCAGAGT187200.027.76126515-19
GAGTACA184700.027.67704220-24
CGCAGAG189250.027.56707215-19
Produced by FastQC (version 0.11.5)