FastQCFastQC Report
Sat 18 Mar 2023
SRR4895400_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895400_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314375
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA1320750.0143.29342
TCTTCAC1323250.0143.022671
TTCACAG455900.0142.727773
TCACATG161400.0142.340684
TTCACAT480850.0141.640443
TCACAGG156850.0141.512484
TCACAGT170700.0141.376174
TCACAGA131900.0141.043624
TCACAAG135900.0140.918494
CACATAG51150.0140.472295
TCACATA142700.0140.055854
TTCACAA400100.0139.672473
TCACAAT148550.0139.435384
CACATGG60900.0139.380585
CACATGT58400.0138.813375
TCACATT180450.0138.804434
CACATTG63100.0138.628545
CACAGGT57100.0138.191155
CACATGA45900.0138.030725
CACAATG52300.0137.521165
Produced by FastQC (version 0.11.5)