FastQCFastQC Report
Sat 18 Mar 2023
SRR4895400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314375
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76070.578754160722777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG47910.36450784593437946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC33550.25525439847836423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21180.16114122681883025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT18220.13862101759391346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT326950.0138.840681
GGTATCA328950.0137.81628
GTGGTAT329600.0137.478886
GTATCAA331700.0137.06439
TGGTATC330800.0137.02377
CAGTGGT332750.0136.199074
AGCAGTG332350.0136.17322
AGTGGTA332800.0136.156985
GCAGTGG337250.0134.194723
ATCAACG328050.028.23140310-14
TCAACGC329800.028.12526110-14
AGAGTAC330350.027.98732815-19
CAACGCA332850.027.9410810-14
CAGAGTA331300.027.87665215-19
GTACATG330100.027.80083820-24
TACATGG331350.027.68292220-24
CGCAGAG335950.027.5336615-19
GCAGAGT335200.027.51794415-19
AACGCAG338650.027.47954410-14
AGTACAT336050.027.40287620-24
Produced by FastQC (version 0.11.5)