FastQCFastQC Report
Sat 18 Mar 2023
SRR4895399_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895399_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100568
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA1102150.0143.785662
CGTCTAA326500.0143.685973
CGTCTAG374750.0143.648863
CGTCTAT401200.0143.402483
GTCTAAG117800.0143.322394
TCCGTCT1105900.0143.298081
GTCTAGA103200.0143.087744
GTCTAGG134900.0142.286824
GTCTAGT138750.0142.230424
GTCTATA119350.0141.883324
GTCTATG139700.0141.830124
GTCTAAA89900.0141.592124
GTCTAAT121450.0141.504854
GTCTATT147400.0140.4294
TCTAGGA42300.0140.420355
TCTAGAG34900.0140.075065
TCTAAGG42450.0139.754555
TCTAGTA39400.0139.426335
CTAGGTA14050.0139.389086
CTATGGA13750.0139.288456
Produced by FastQC (version 0.11.5)