FastQCFastQC Report
Sat 18 Mar 2023
SRR4895398_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895398_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891730
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC1893600.0143.848431
ATCAGCA1893850.0143.829442
TCAGCAG659800.0143.611013
TCAGCAT677800.0143.536163
TCAGCAA559100.0143.104393
CAGCATG234500.0142.918334
CAGCAGT242750.0142.865894
CAGCAAT202700.0142.856314
CAGCATA196950.0142.53074
CAGCATT249450.0142.26124
CAGCAAG196250.0142.085244
AGCAATG72150.0141.79765
CAGCAGG235300.0141.789894
CAGCAGA187150.0141.415444
AGCATAG68500.0141.365315
AGCATGT85200.0140.95085
AGCAGTA74000.0140.782275
AGCAGTG82200.0140.489445
AGCATGA64400.0140.415455
AGCAAGT71950.0140.39065
Produced by FastQC (version 0.11.5)