FastQCFastQC Report
Sat 18 Mar 2023
SRR4895398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891730
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109880.5808439893642328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA25390.13421577074952556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT23840.12602221247218154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23010.12163469416882958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22170.11719431419917219No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20850.11021657424685341No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT19180.10138867597384404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC18950.10017285764881881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT448450.0138.219971
GGTATCA449000.0137.758388
GTGGTAT449150.0137.696336
AGTGGTA450000.0137.484245
TGGTATC450600.0137.173347
GTATCAA453650.0136.663749
CAGTGGT456350.0135.586964
AGCAGTG458000.0135.05492
GCAGTGG462050.0133.886693
ATCAACG449200.028.12603410-14
TCAACGC450500.028.07683610-14
AGAGTAC451650.027.96100615-19
CAGAGTA451950.027.92332815-19
CAACGCA455950.027.82334310-14
TACATGG450500.027.79382920-24
GTACATG453150.027.73615820-24
AGTACAT456550.027.58637820-24
AACGCAG460600.027.52682110-14
GCAGAGT457400.027.5024715-19
CGCAGAG460850.027.3715715-19
Produced by FastQC (version 0.11.5)