FastQCFastQC Report
Sat 18 Mar 2023
SRR4895397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291236
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108930.47542025352255285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG76200.33257159018102017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC71230.31088024105766493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT29670.12949342625552324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA27080.11818948375461977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT439050.0138.308091
GGTATCA439000.0138.107628
GTGGTAT442050.0137.122156
GTATCAA443750.0136.969999
TGGTATC443500.0136.65767
CAGTGGT451400.0134.265934
AGCAGTG451250.0134.185872
AGTGGTA453650.0133.615895
GCAGTGG456250.0132.794223
ATCAACG440350.028.13852710-14
TCAACGC441350.028.10413410-14
CAACGCA443450.028.05222310-14
AGAGTAC442850.027.9602915-19
TACATGG439400.027.90175620-24
GTACATG441450.027.82437720-24
CAGAGTA445350.027.80009815-19
AGTACAT447750.027.58081620-24
CGCAGAG448250.027.57205615-19
GAGTACA441700.027.35547620-24
GCAGAGT451700.027.34871515-19
Produced by FastQC (version 0.11.5)