FastQCFastQC Report
Sat 18 Mar 2023
SRR4895396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405706
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG1409250.0143.633241
AGGATGA1410100.0143.546662
GGATGAA433700.0142.999943
GGATGAT552350.0142.953193
GGATGAG429300.0142.671073
GATGATA174200.0137.54844
GATGATG186700.0136.861744
GATGAAG143400.0136.063144
GATGAAT171850.0135.82634
GATGAGA131350.0135.061334
GATGAGG150150.0134.981254
GATGAAA141350.0134.725634
GATGATT218350.0134.169834
GATGAGT172450.0133.808964
ATGATGA54200.0132.571935
ATGATGG63550.0131.986885
ATGATAG60600.0131.63995
ATGAATG56950.0130.595125
ATGATTG76200.0130.390065
ATGAAGG46800.0130.304065
Produced by FastQC (version 0.11.5)