FastQCFastQC Report
Sat 18 Mar 2023
SRR4895396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405706
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68160.48488090681835316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG29750.21163742631816326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21170.15060048118169803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19130.1360882005198811No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT16990.1208645335511124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT368650.0139.546971
GGTATCA370600.0138.613488
GTATCAA372000.0138.362789
GTGGTAT371150.0138.330496
TGGTATC373350.0137.573237
AGCAGTG374400.0137.192262
AGTGGTA376100.0136.605585
CAGTGGT376450.0136.459464
GCAGTGG378950.0135.55923
CGTGGTA1453.6611484E-529.7920364
ATCAACG370500.028.30149810-14
TCAACGC372000.028.21834410-14
CAACGCA374100.028.16771710-14
CAGAGTA372600.028.13039215-19
AGAGTAC372800.028.11530115-19
TACATGG369900.028.00912720-24
GTACATG370800.027.97609520-24
AGTACAT374400.027.87247520-24
CGCAGAG377950.027.7436315-19
GCAGAGT377050.027.73729315-19
Produced by FastQC (version 0.11.5)