FastQCFastQC Report
Sat 18 Mar 2023
SRR4895395_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895395_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816166
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCTA2828700.0143.355162
GTTCTAG946600.0143.218223
GTTCTAA803500.0143.0893
GTTCTAT1083000.0142.750843
GTGTTCT2850500.0142.258821
TTCTAGA255550.0140.866784
TTCTAGG331850.0140.804754
TCTAGGA87900.0140.144335
TCTAGAG80850.0139.986765
TCTAGAT100650.0139.91675
TCTATAG108250.0139.870395
TTCTAGT375800.0139.81754
TTCTATG387100.0139.734834
TCTAGTA107400.0139.569635
TTCTAAG276550.0139.412034
TCTATGG139900.0139.207935
TCTATGA100550.0139.1255
TCTAAGG98400.0138.945435
TCTAGGT131450.0138.84555
TCTAGTG124150.0138.600775
Produced by FastQC (version 0.11.5)