FastQCFastQC Report
Sat 18 Mar 2023
SRR4895395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816166
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126730.45000898384541255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG117580.4175180014246319No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC78460.27860573559939295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT50170.17815000962301228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA41680.14800263904897654No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC30400.10794818203188307No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC28760.10212466168542621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT726150.0138.423951
GTGGTAT724750.0138.226976
GGTATCA725600.0138.11228
TGGTATC725650.0138.025777
GTATCAA731700.0137.393759
CAGTGGT736600.0136.081454
AGTGGTA738200.0135.796255
AGCAGTG738400.0135.717962
GCAGTGG748750.0133.822683
CAACGCA727750.028.2214910-14
TCAACGC726250.028.21038410-14
ATCAACG725850.028.1783210-14
AGAGTAC729700.027.99619915-19
CAGAGTA730900.027.93053215-19
TACATGG727600.027.87355220-24
CGCAGAG733550.027.84729815-19
GTACATG729800.027.82701920-24
AGTACAT736700.027.66607520-24
AACGCAG744000.027.63025510-14
GCAGAGT737450.027.62582815-19
Produced by FastQC (version 0.11.5)