FastQCFastQC Report
Sat 18 Mar 2023
SRR6792046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106192000
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24052162.264969112550851No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG8906120.8386808799156246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7461400.7026329667018231No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC4734110.4458066521018532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA4449160.41897318065390987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4439110.4180267816784692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG3659730.34463330571041134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT2651790.24971655115262917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2609530.24573696700316408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA1956430.18423515895736026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG1687450.15890556727437094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA1329450.12519304655717944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC1265840.11920295314147959No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCTCCTCGTTGGGCG1248480.11756817839385265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCTCCTCGTTGGGCGT1139690.10732352719602231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT42268400.0110.481531
AGTGGTA42320000.0110.069325
GTATCAA42558550.0109.670259
GTGGTAT42552400.0109.4359746
GGTATCA42588050.0109.418198
TGGTATC42882100.0108.596567
AGCAGTG43091450.0108.189362
GCAGTGG43480100.0107.110873
CAGTGGT43503050.0107.083194
TAGTACC787050.090.56858145
CCCTCTT3292650.046.292194145
TGCGGAT303650.042.6906145
CCTCTTC2353050.035.066086145
TGACTGG1057500.032.1410332
CGGTCTG597750.031.838144145
AGTTCAG1078550.031.423069
GTGACTG1146800.029.8624521
ACGGTCT714400.028.38501145
GACTGGA1205650.028.229853
CATGGGG14744700.025.51676825-29
Produced by FastQC (version 0.11.5)