FastQCFastQC Report
Sat 18 Mar 2023
SRR6792045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82472965
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1148767113.929014192711515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13560101.6441872800377673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG3882230.4707275893379121No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3382060.4100810489837488No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG2923430.354471310689509No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2891180.3505609383632564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC2129830.2582458384999254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA1986740.24089591055687157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA1591150.1929298892067237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1432570.17370177002851783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1201190.14564651580066268No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1095650.13284959501577276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT941030.11410163318367418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC418600.084.12089145
AAGCAGT80977100.064.890561
GTATCAA81010800.064.8506249
AGTGGTA81181450.064.634295
GGTATCA82106200.063.9126558
GTGGTAT82119800.063.8726966
AGCAGTG82275050.063.7971152
TGGTATC82365350.063.658057
CAGTGGT83064450.063.1694374
GCAGTGG84656100.061.9601253
CGTGGGG462100.047.96168145
CAGGGGA1745050.036.06175145
CCCTCTT1570450.035.74078145
TGCGGAT141500.030.38318145
CAGGGGG672550.030.312777145
CACGGGG422500.029.463106145
CGTGGGA1434550.028.730934145
CACGGGA807700.028.41815145
TGACTGG549650.027.9586582
GACTGGA594900.026.0352973
Produced by FastQC (version 0.11.5)