FastQCFastQC Report
Sat 18 Mar 2023
SRR6792042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92905271
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG12588681.3550016984504571No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12264421.3200994806850088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12023821.2942021341286438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA6035760.6496681980508943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC4634920.49888665628024487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT4597360.494843828613341No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG2099680.2260022469553961No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1880320.2023911000700918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG1673160.1800931187208958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC1413750.1521711292354984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA1037860.11171163797584747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA960910.10342900781162352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT27067750.0112.330451
GTGGTAT27219750.0111.07566
AGTGGTA27271150.0110.985885
GGTATCA27292400.0110.875828
GTATCAA27442000.0110.636229
TGGTATC27385900.0110.29747
AGCAGTG27836650.0108.882062
GCAGTGG27899850.0108.487023
CAGTGGT28002100.0108.087264
TAGTACC771600.092.99906145
CCCTCTT3851750.053.303837145
CCTCTTC2319450.047.293987145
TGACTGG920900.039.310712
AGTTCAG946850.038.0234649
TGCGGAT239400.037.70236145
GTGACTG996100.036.50521
GACTGGA1026750.035.341813
CGGTCTG490150.027.09691145
TGGAGTT1395250.026.0842176
ACCCGAC3216950.025.58874960-64
Produced by FastQC (version 0.11.5)