FastQCFastQC Report
Sat 18 Mar 2023
SRR6792041_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792041_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98297000
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2427710.2469770186272216No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATAC1875010.19074946336103848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA4178300.0141.818052
ACGTGAT2902550.0141.25612
AGATCGC4273600.0140.923071
GATAGAC2213100.0140.85621
AACGTGA2899500.0140.822541
GCTAACG1639150.0140.474521
ACGTATC1751950.0140.290011
ATCGCAT2374550.0140.222413
TATCAGC2337950.0140.18761
AACATCG2956200.0139.896912
CTAACGA1618050.0139.878722
ATGCCTA1711850.0139.788251
CCGTCTA3388050.0138.664812
GCGAGTA1633950.0138.617651
GACTAGT1874800.0138.223711
ATCAGCA2319350.0138.092212
AGTCACT1750600.0138.01161
GTCACTA1715550.0137.952092
CGAACTT1790500.0137.950071
AAGGTAC2061500.0137.232071
Produced by FastQC (version 0.11.5)