FastQCFastQC Report
Sat 18 Mar 2023
SRR6792038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78941170
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3817180.48354743158734537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2668520.33803907390782273No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATAC1720120.21789897464149569No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT1237160.156719237883097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTA2005850.0141.425022
ACGCTTA1711550.0141.030622
ACGTGAT1975950.0141.026032
ACGTATC1540100.0140.981281
GCTAACG1370300.0140.976361
CTAACGA1350600.0140.694552
AACGTGA1976100.0140.653751
ACTTCGA1643550.0140.248842
CGAACTT1841350.0139.301271
ACAAGCT2068350.0139.02251
TATCAGC1459050.0138.723251
GAACTTA1825450.0138.680832
ATGCCTA996250.0138.418731
AGTACAA2636850.0138.325011
GACTAGT1491150.0138.207461
AACATCG1450500.0137.852142
CCGTCTA2232850.0137.593342
ACTAGTA1487900.0137.257062
GCGAGTA1123950.0137.131671
TGGCATA1401000.0137.118352
Produced by FastQC (version 0.11.5)