FastQCFastQC Report
Thu 2 Feb 2017
SRR4064248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589565
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29300.18432715868806876No Hit
TATCAACGCAGAGTACTTTTTTTTT18900.11890045389776449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC954.9221853E-913.9957321
GCTATAT500.001503021313.2959461
GTATCAA16300.012.0024761
TCGCGTA802.8643151E-511.8758669
ATCCCCG1701.8189894E-1211.7357812
CGGTCGG1302.6120688E-911.6931610
TCCGTCA2300.011.56715417
CCGTCAA2350.011.32175718
GCGCTAC1104.9874507E-711.2238511
GTCCTAC5700.011.1632621
GGGTATA600.00588910211.0799551
GGTATAC600.00588910211.0799551
ACGGTAT2000.010.9257979
CGTCAAT2450.010.85964419
CGCCAGT1408.3182385E-910.85964418
TCGGCGT1408.341885E-910.85759313
GTCTAGG1158.8497654E-710.7358571
AAGACGG2750.010.7075125
GTTATAT803.7814115E-410.6842421
GTGTAGA2850.010.6634151
Produced by FastQC (version 0.11.5)