##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064248_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1589565
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215357660743663	32.0	32.0	32.0	32.0	32.0
2	30.801618052737698	32.0	32.0	32.0	32.0	32.0
3	30.819736846244098	32.0	32.0	32.0	32.0	32.0
4	30.84844155476498	32.0	32.0	32.0	32.0	32.0
5	30.750064325774662	32.0	32.0	32.0	32.0	32.0
6	34.39198774507491	36.0	36.0	36.0	32.0	36.0
7	34.33998924233988	36.0	36.0	36.0	32.0	36.0
8	34.28788882493009	36.0	36.0	36.0	32.0	36.0
9	34.44374467228456	36.0	36.0	36.0	32.0	36.0
10	34.130818179816494	36.0	36.0	36.0	32.0	36.0
11	34.41607169256998	36.0	36.0	36.0	32.0	36.0
12	34.23629672268828	36.0	36.0	36.0	32.0	36.0
13	34.31068688603486	36.0	36.0	36.0	32.0	36.0
14	34.22006209245926	36.0	36.0	36.0	32.0	36.0
15	34.14738371818705	36.0	36.0	36.0	32.0	36.0
16	34.13979296222551	36.0	36.0	36.0	32.0	36.0
17	34.08069125830023	36.0	36.0	36.0	32.0	36.0
18	34.07546215474045	36.0	36.0	36.0	32.0	36.0
19	34.089567900652064	36.0	36.0	36.0	32.0	36.0
20	34.06568274968309	36.0	36.0	36.0	32.0	36.0
21	34.0568891489181	36.0	36.0	36.0	32.0	36.0
22	34.031669670633164	36.0	36.0	36.0	32.0	36.0
23	33.97177970073574	36.0	36.0	36.0	32.0	36.0
24	33.94585185255085	36.0	36.0	36.0	32.0	36.0
25	33.543962656450034	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	12.0
5	23.0
6	56.0
7	28.0
8	97.0
9	102.0
10	148.0
11	37.0
12	104.0
13	83.0
14	108.0
15	253.0
16	435.0
17	590.0
18	739.0
19	1006.0
20	1504.0
21	2277.0
22	3539.0
23	5607.0
24	8472.0
25	12536.0
26	18105.0
27	24324.0
28	33542.0
29	45084.0
30	60068.0
31	80818.0
32	113956.0
33	160475.0
34	334249.0
35	681183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.915137158551104	16.823477435612137	11.07350449726992	26.18788090856684
2	16.49982006739294	19.403529698946326	37.99365076968375	26.10299946397699
3	19.005914911339243	23.475504264750473	28.637305588653838	28.881275235256442
4	12.46178865880473	15.088627663155114	36.15530523461559	36.29427844342457
5	14.299906193199227	36.80259110615897	34.15750356887376	14.739999131768041
6	34.51059446937051	35.63550772099836	16.68727235433981	13.16662545529132
7	30.19619218893448	30.419196492338628	20.796283932804222	18.588327385922664
8	27.749629485118042	33.96968543207856	19.220140905786955	19.060544177016432
9	27.33991946092445	13.841693762690443	18.254411836902705	40.5639749394824
10	15.329212408097492	27.12810957800383	32.45115318763219	25.091524826266493
11	37.49775761607995	20.707605096206922	22.682980101478496	19.111657186234627
12	24.423653891430003	23.382384601729132	29.579639694764133	22.614321812076735
13	29.661326366812503	19.55525479210601	25.231752226873567	25.551666614207925
14	23.01424328533532	19.671484094179682	25.418907196428414	31.89536542405659
15	24.890471250591705	27.89181077460746	22.154015046984057	25.063702927816777
16	25.273160490640752	25.79735416594535	24.363202573440308	24.56628276997359
17	23.860347971801176	26.128226708557406	25.526115510925816	24.4853098087156
18	24.387693365374385	25.22924314662936	26.564692683547186	23.818370804449064
19	25.486269374598656	24.891338562848617	25.265295073091497	24.357096989461226
20	25.502500066106006	24.79932105986182	25.10221247351038	24.595966400521796
21	26.13081492710959	24.50397000274488	24.61899053897855	24.746224531166977
22	25.54450645789853	24.836168009242208	24.973856764660514	24.645468768198743
23	24.330380472080034	24.583797669191394	25.49320338225387	25.592618476474698
24	24.705986048502858	25.039033972147372	25.47923644513838	24.77574353421139
25	24.671946249739516	24.798364122506978	25.57443639223034	24.95525323552317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	84.0
2	254.5
3	425.0
4	425.0
5	425.0
6	1188.5
7	1952.0
8	1952.0
9	1952.0
10	2262.5
11	2573.0
12	2573.0
13	2573.0
14	3372.0
15	4171.0
16	4171.0
17	4171.0
18	7121.5
19	10072.0
20	10072.0
21	10072.0
22	16410.0
23	22748.0
24	22748.0
25	22748.0
26	34487.0
27	46226.0
28	46226.0
29	46226.0
30	60574.0
31	74922.0
32	74922.0
33	74922.0
34	89788.5
35	104655.0
36	104655.0
37	104655.0
38	119183.5
39	133712.0
40	133712.0
41	133712.0
42	151338.5
43	168965.0
44	168965.0
45	168965.0
46	186869.0
47	204773.0
48	204773.0
49	204773.0
50	208209.5
51	211646.0
52	211646.0
53	211646.0
54	199157.5
55	186669.0
56	186669.0
57	186669.0
58	170487.0
59	154305.0
60	154305.0
61	154305.0
62	134555.5
63	114806.0
64	114806.0
65	114806.0
66	93717.5
67	72629.0
68	72629.0
69	72629.0
70	54412.0
71	36195.0
72	36195.0
73	36195.0
74	27646.5
75	19098.0
76	19098.0
77	19098.0
78	15038.0
79	10978.0
80	10978.0
81	10978.0
82	7495.0
83	4012.0
84	4012.0
85	4012.0
86	3005.0
87	1998.0
88	1998.0
89	1998.0
90	1404.0
91	810.0
92	810.0
93	810.0
94	573.0
95	336.0
96	336.0
97	336.0
98	570.5
99	805.0
100	805.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03780908613362775
2	0.005095733738475621
3	0.0014469367405548059
4	0.0031455146533800127
5	0.008052517512652834
6	0.012456238027384851
7	0.02252188491820089
8	0.035229764117856145
9	0.0485038359551198
10	0.05819202108753024
11	0.053725390279730625
12	0.06435722980815506
13	0.06385394746361427
14	0.06460887098042546
15	0.05919858577661184
16	0.06479760185962825
17	0.06215536955078905
18	0.07461160757817391
19	0.07203228556240229
20	0.07593272373259351
21	0.07253556790694309
22	0.07549235168112031
23	0.08021062366119033
24	0.07580690314645831
25	0.0743599664059035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1589565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.441654590415325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76557251185736	45.81866464432838
2	11.986928665071128	13.770980319579278
3	3.5480477167963	6.1141719425557435
4	1.5144040235450316	3.4795949132323556
5	0.8264627892471699	2.3736695035883573
6	0.521919021841399	1.7987935306068643
7	0.33960408655680807	1.3655194446242773
8	0.23367148618180933	1.0737981437507598
9	0.1688170662409957	0.8727418447184285
>10	0.95963035499459	9.713270471758458
>50	0.06809117173871283	2.7171708901287963
>100	0.05848115412496204	6.9796298463596935
>500	0.00660785668717507	2.6054690658160937
>1k	0.0017620951165800186	1.3165254389525256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2930	0.18432715868806876	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1890	0.11890045389776449	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.291029306760025E-5
2	0.0	0.0	0.0	0.0	6.291029306760025E-5
3	0.0	0.0	0.0	0.0	6.291029306760025E-5
4	0.0	0.0	0.0	0.0	6.291029306760025E-5
5	0.0	0.0	0.0	0.0	6.291029306760025E-5
6	0.0	0.0	0.0	0.0	6.291029306760025E-5
7	0.0	0.0	0.0	6.291029306760025E-5	6.291029306760025E-5
8	0.0	0.0	0.0	1.258205861352005E-4	1.258205861352005E-4
9	0.0	0.0	0.0	1.8873087920280078E-4	1.258205861352005E-4
10	0.0	0.0	0.0	1.8873087920280078E-4	1.258205861352005E-4
11	0.0	0.0	0.0	1.8873087920280078E-4	1.258205861352005E-4
12	0.0	0.0	0.0	2.51641172270401E-4	3.145514653380013E-4
13	0.0	0.0	0.0	2.51641172270401E-4	4.403720514732018E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	95	4.9221853E-9	13.995732	1
GCTATAT	50	0.0015030213	13.295946	1
GTATCAA	1630	0.0	12.002476	1
TCGCGTA	80	2.8643151E-5	11.875866	9
ATCCCCG	170	1.8189894E-12	11.73578	12
CGGTCGG	130	2.6120688E-9	11.69316	10
TCCGTCA	230	0.0	11.567154	17
CCGTCAA	235	0.0	11.321757	18
GCGCTAC	110	4.9874507E-7	11.223851	1
GTCCTAC	570	0.0	11.163262	1
GGGTATA	60	0.005889102	11.079955	1
GGTATAC	60	0.005889102	11.079955	1
ACGGTAT	200	0.0	10.925797	9
CGTCAAT	245	0.0	10.859644	19
CGCCAGT	140	8.3182385E-9	10.859644	18
TCGGCGT	140	8.341885E-9	10.857593	13
GTCTAGG	115	8.8497654E-7	10.735857	1
AAGACGG	275	0.0	10.707512	5
GTTATAT	80	3.7814115E-4	10.684242	1
GTGTAGA	285	0.0	10.663415	1
>>END_MODULE