FastQCFastQC Report
Thu 2 Feb 2017
SRR4064248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589565
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT300.00574081329.33828530
GTATCAA21150.022.9862731
TATACCG703.218234E-521.9981755
TATACTG2200.020.9982595
CGTGACG550.004483110320.00085610
TACGACG3750.018.7717765
GATATAC4600.018.6506271
TGTAGGA10450.018.5241992
CGACGGT3700.018.4309047
CGTAAAT600.007417993618.33123816
GTAGGAC9800.017.957133
TAGGACG7850.017.9348184
CGCAATA3700.017.83972536
GTACCGT750.00129183117.598546
AACGCAG27350.017.5341956
TACGAAT3900.017.48627941
GCGAAAG3900.017.4857318
AGGACGT8400.017.284285
TATGGCG9450.016.99276416
CTGTAGG10300.016.8723891
Produced by FastQC (version 0.11.5)