##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1589565
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291608081456246	32.0	32.0	32.0	32.0	32.0
2	31.331771270756466	32.0	32.0	32.0	32.0	32.0
3	31.432016935450893	32.0	32.0	32.0	32.0	32.0
4	31.523565881231658	32.0	32.0	32.0	32.0	32.0
5	31.47489470389698	32.0	32.0	32.0	32.0	32.0
6	35.04895364455055	36.0	36.0	36.0	36.0	36.0
7	35.071190545841155	36.0	36.0	36.0	36.0	36.0
8	35.00795815207305	36.0	36.0	36.0	36.0	36.0
9	35.13200844256133	36.0	36.0	36.0	36.0	36.0
10	34.96780377021386	36.0	36.0	36.0	36.0	36.0
11	35.126125071953645	36.0	36.0	36.0	36.0	36.0
12	35.03211696281687	36.0	36.0	36.0	36.0	36.0
13	35.073298669761854	36.0	36.0	36.0	36.0	36.0
14	35.018114389785886	36.0	36.0	36.0	36.0	36.0
15	34.98533686889181	36.0	36.0	36.0	36.0	36.0
16	35.00470820633318	36.0	36.0	36.0	36.0	36.0
17	34.97007168627895	36.0	36.0	36.0	36.0	36.0
18	34.97083415903093	36.0	36.0	36.0	36.0	36.0
19	34.961237193823465	36.0	36.0	36.0	36.0	36.0
20	34.958889381686184	36.0	36.0	36.0	36.0	36.0
21	34.94732646982036	36.0	36.0	36.0	36.0	36.0
22	34.93368877648916	36.0	36.0	36.0	36.0	36.0
23	34.87836043194207	36.0	36.0	36.0	32.0	36.0
24	34.847949596273196	36.0	36.0	36.0	32.0	36.0
25	34.82133350948215	36.0	36.0	36.0	32.0	36.0
26	34.76920478244048	36.0	36.0	36.0	32.0	36.0
27	34.75485557369469	36.0	36.0	36.0	32.0	36.0
28	34.73540874390164	36.0	36.0	36.0	32.0	36.0
29	34.70520614130281	36.0	36.0	36.0	32.0	36.0
30	34.6839021996584	36.0	36.0	36.0	32.0	36.0
31	34.67705944708143	36.0	36.0	36.0	32.0	36.0
32	34.64424418001151	36.0	36.0	36.0	32.0	36.0
33	34.620593684435676	36.0	36.0	36.0	32.0	36.0
34	34.61470339369576	36.0	36.0	36.0	32.0	36.0
35	34.57829972350926	36.0	36.0	36.0	32.0	36.0
36	34.55429567208639	36.0	36.0	36.0	32.0	36.0
37	34.54237228424129	36.0	36.0	36.0	32.0	36.0
38	34.49669563685662	36.0	36.0	36.0	32.0	36.0
39	34.484465246781355	36.0	36.0	36.0	32.0	36.0
40	34.478110048975665	36.0	36.0	36.0	32.0	36.0
41	34.465852607474375	36.0	36.0	36.0	32.0	36.0
42	34.4137547064763	36.0	36.0	36.0	32.0	36.0
43	34.403271335239516	36.0	36.0	36.0	32.0	36.0
44	34.331629722597064	36.0	36.0	36.0	32.0	36.0
45	34.3064240845766	36.0	36.0	36.0	32.0	36.0
46	34.288105865441175	36.0	36.0	36.0	32.0	36.0
47	34.27080805125931	36.0	36.0	36.0	32.0	36.0
48	34.22107117356006	36.0	36.0	36.0	32.0	36.0
49	34.21099294461063	36.0	36.0	36.0	32.0	36.0
50	33.59952313997855	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	16.0
21	45.0
22	148.0
23	473.0
24	1272.0
25	2941.0
26	5902.0
27	10665.0
28	18145.0
29	28331.0
30	41073.0
31	59481.0
32	86123.0
33	140544.0
34	290209.0
35	904196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68617152463546	17.797417416208216	11.689387800298977	26.82702325885734
2	15.730483398833764	20.195501010658944	37.483147820430474	26.59086777007682
3	18.240948286979865	24.01203854397535	28.964412186760995	28.78260098228379
4	12.084375285062265	15.804323824442537	36.28565047670275	35.82565041379245
5	13.987097098891836	37.247800498878625	33.769742036343274	14.995360365886265
6	34.65761930495275	35.89104161463448	16.390689001554517	13.060650078858252
7	30.252301730347607	30.410709848291827	20.811479870278976	18.525508551081586
8	27.808803037308948	34.14437283156084	19.054835756952375	18.99198837417784
9	27.30599565160498	13.730912860982214	18.084844031525865	40.87824745588694
10	15.137394490379963	27.34118588503517	32.530840547652886	24.990579076931983
11	37.72386641871607	20.58432375167027	22.64287565646932	19.048934173144332
12	24.44406139041901	23.321174863044206	29.572889169608775	22.661874576928014
13	29.788576580045156	19.444638418580247	25.20573316246833	25.561051838906263
14	23.001479022850933	19.58959789577387	25.36300857531284	32.04591450606236
15	24.894259134481995	28.04021896207547	22.02914251597516	25.036379387467374
16	25.388772818521936	25.823171671532812	24.244551770214645	24.543503739730603
17	23.882634557253084	26.216040237423442	25.397451504027828	24.503873701295635
18	24.478252499745214	25.294640913660494	26.434137202575304	23.792969384018996
19	25.535870213467344	24.88789378722719	25.256107964196474	24.320128035108997
20	25.64000777578071	24.7732221237318	25.021279594048885	24.56549050643861
21	26.29562175815396	24.54463957120344	24.444738025812093	24.715000644830504
22	25.61370640861671	24.735792164261497	25.002154680248594	24.648346746873198
23	24.37636830317824	24.51936384911548	25.504101764010166	25.60016608369612
24	24.818612984624018	25.022065896347222	25.307428421881067	24.851892697147697
25	24.778881874258822	24.840220567650288	25.438068365905753	24.942829192185137
26	24.34729269359136	25.531001984907153	25.798508331262443	24.323196990239037
27	24.90454383308178	25.141729365645094	25.09982868535672	24.853898115916408
28	24.325810427297622	25.303727396207993	25.394699342749703	24.975762833744678
29	24.481402981637956	24.924490210622142	25.287742469624654	25.30636433811525
30	24.38009509801084	25.326482619259423	25.526102881502442	24.76731940122729
31	25.130390031343943	24.861179443107165	24.845954165623336	25.162476359925563
32	24.605053478442223	25.259654021122113	24.899985467301743	25.235307033133925
33	24.302100555903678	24.858130059818155	25.514506594592962	25.325262789685205
34	24.93781149301488	25.05375829966493	25.609711598832856	24.398718608487332
35	25.17610673417556	25.01863771367313	25.498720053047496	24.306535499103816
36	24.40395936448337	25.30269961816909	25.311444798096446	24.981896219251098
37	25.05849305286866	25.118385912595432	25.03301342241767	24.790107612118238
38	24.61562078862698	25.135487459152433	25.249741104762286	24.999150647458297
39	25.24065759085095	24.732447142149823	24.952076094417173	25.074819172582053
40	25.540347724990752	24.971249399194228	25.341798718113257	24.146604157701766
41	24.622905976883647	24.97030557575727	25.84849300796968	24.5582954393894
42	25.372103337747593	25.50805419160829	25.200607721077418	23.9192347495667
43	24.54032616757814	24.81147191879971	25.70499468088787	24.943207232734274
44	24.321520533837628	25.281605388201793	25.177739318183107	25.21913475977747
45	24.408400632381426	25.448697018710366	25.210830507056414	24.932071841851798
46	24.444765987965187	24.804426396066823	25.332435807505878	25.418371808462116
47	24.82738749439304	24.86777646153643	25.61207844015544	24.692757603915087
48	25.056494331693447	25.30801363916605	24.85184392969035	24.783648099450158
49	24.642920951908824	25.330971260043633	25.042590295200444	24.983517492847096
50	24.55504506641217	25.763002322051054	24.833867344261844	24.848085267274932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	19.0
2	23.0
3	74.0
4	125.0
5	175.5
6	226.0
7	193.5
8	161.0
9	230.5
10	300.0
11	469.0
12	638.0
13	1125.5
14	1613.0
15	2157.0
16	2701.0
17	2854.5
18	3008.0
19	3160.5
20	3313.0
21	4092.5
22	4872.0
23	5847.0
24	6822.0
25	8569.5
26	10317.0
27	13312.0
28	16307.0
29	19400.0
30	22493.0
31	25639.0
32	28785.0
33	33738.0
34	38691.0
35	45265.5
36	51840.0
37	58088.0
38	64336.0
39	68393.0
40	72450.0
41	77311.5
42	82173.0
43	84826.0
44	87479.0
45	95118.0
46	102757.0
47	108206.0
48	113655.0
49	117432.0
50	121209.0
51	118746.0
52	116283.0
53	115104.5
54	113926.0
55	113076.5
56	112227.0
57	108147.5
58	104068.0
59	95423.0
60	86778.0
61	76430.5
62	66083.0
63	57199.0
64	48315.0
65	41134.5
66	33954.0
67	28933.0
68	23912.0
69	21508.0
70	19104.0
71	14722.5
72	10341.0
73	8825.5
74	7310.0
75	5693.5
76	4077.0
77	3456.0
78	2835.0
79	2336.0
80	1837.0
81	1424.5
82	1012.0
83	809.5
84	607.0
85	442.0
86	277.0
87	197.0
88	117.0
89	94.5
90	72.0
91	56.5
92	41.0
93	32.0
94	23.0
95	23.0
96	23.0
97	18.5
98	14.0
99	17.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009373633667072438
2	5.032823445408021E-4
3	2.5164117227040105E-4
4	0.0
5	0.0
6	2.5164117227040105E-4
7	0.0
8	0.0
9	8.178338098788032E-4
10	0.0011323852752168047
11	6.291029306760026E-5
12	6.291029306760026E-5
13	3.774617584056015E-4
14	1.2582058613520053E-4
15	0.004152079342461617
16	7.54923516811203E-4
17	0.0
18	1.8873087920280075E-4
19	6.291029306760026E-5
20	8.807441029464036E-4
21	0.0
22	1.2582058613520053E-4
23	3.1455146533800125E-4
24	5.032823445408021E-4
25	0.0018873087920280075
26	0.004403720514732018
27	0.005787746962219223
28	0.004026258756326417
29	0.0022647705504336094
30	0.0029567837741772116
31	0.006479760185962827
32	0.0028938734811096117
33	0.005095733738475621
34	0.0027051426019068106
35	0.0028938734811096117
36	0.007423414581976829
37	0.003774617584056015
38	0.007486324875044431
39	0.004152079342461617
40	0.0020760396712308084
41	0.0025793220157716104
42	0.001258205861352005
43	8.807441029464036E-4
44	0.0011323852752168047
45	0.0011323852752168047
46	6.291029306760025E-4
47	0.0013840264474872055
48	0.0015727573266900064
49	6.291029306760026E-5
50	0.0013840264474872055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1589565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.3131666146297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88596550258616	49.59374679645255
2	11.660033446270516	14.29827146846678
3	3.274150775849901	6.022456561233123
4	1.3638337666986193	3.3448386788900186
5	0.7337898415342923	2.2495489407057394
6	0.46450221554105364	1.7088061040599956
7	0.31777864264370465	1.3638810404089032
8	0.21190817515250757	1.0394209000102266
9	0.15371500122058496	0.848227813290516
>10	0.831129892610713	8.9123512981687
>50	0.05258262883754328	2.282847972842478
>100	0.045147267871909896	5.82371040976918
>500	0.004535190566567374	1.8653864852056767
>1k	9.276526158887812E-4	0.6465055304961234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.291029306760025E-5
6	0.0	0.0	0.0	0.0	1.258205861352005E-4
7	0.0	0.0	0.0	6.291029306760025E-5	1.258205861352005E-4
8	0.0	0.0	0.0	1.258205861352005E-4	1.258205861352005E-4
9	0.0	0.0	0.0	1.258205861352005E-4	1.8873087920280078E-4
10	0.0	0.0	0.0	1.258205861352005E-4	1.8873087920280078E-4
11	0.0	0.0	0.0	1.258205861352005E-4	1.8873087920280078E-4
12	0.0	0.0	0.0	1.258205861352005E-4	3.145514653380013E-4
13	0.0	0.0	0.0	1.8873087920280078E-4	3.145514653380013E-4
14	0.0	0.0	0.0	1.8873087920280078E-4	3.7746175840560155E-4
15	0.0	6.291029306760025E-5	0.0	2.51641172270401E-4	5.661926376084022E-4
16	0.0	6.291029306760025E-5	0.0	2.51641172270401E-4	6.291029306760026E-4
17	0.0	6.291029306760025E-5	0.0	2.51641172270401E-4	6.291029306760026E-4
18	0.0	6.291029306760025E-5	0.0	3.7746175840560155E-4	6.291029306760026E-4
19	0.0	6.291029306760025E-5	0.0	5.03282344540802E-4	6.291029306760026E-4
20	0.0	6.291029306760025E-5	0.0	6.920132237436029E-4	6.920132237436029E-4
21	0.0	6.291029306760025E-5	0.0	7.549235168112031E-4	8.178338098788034E-4
22	0.0	6.291029306760025E-5	0.0	8.807441029464036E-4	8.178338098788034E-4
23	0.0	6.291029306760025E-5	0.0	0.0011952955682844049	8.178338098788034E-4
24	0.0	6.291029306760025E-5	0.0	0.0018243984989604074	8.807441029464036E-4
25	0.0	6.291029306760025E-5	0.0	0.0023276808435012094	8.807441029464036E-4
26	0.0	6.291029306760025E-5	0.0	0.002956783774177212	8.807441029464036E-4
27	0.0	6.291029306760025E-5	0.0	0.003963348463258816	9.436543960140039E-4
28	0.0	6.291029306760025E-5	0.0	0.006479760185962826	9.436543960140039E-4
29	0.0	6.291029306760025E-5	0.0	0.012959520371925652	9.436543960140039E-4
30	0.0	6.291029306760025E-5	0.0	0.0241575525379585	9.436543960140039E-4
31	0.0	6.291029306760025E-5	0.0	0.04479212866413138	0.0011323852752168045
32	0.0	6.291029306760025E-5	0.0	0.07027079735650948	0.0011952955682844049
33	0.0	6.291029306760025E-5	0.0	0.09568655575581998	0.0011952955682844049
34	0.0	6.291029306760025E-5	0.0	0.1228638023610233	0.0013211161544196054
35	0.0	6.291029306760025E-5	0.0	0.15551424446310783	0.0013211161544196054
36	0.0	6.291029306760025E-5	0.0	0.198293243749076	0.0013211161544196054
37	0.0	6.291029306760025E-5	0.0	0.26340539707404226	0.0013211161544196054
38	0.0	6.291029306760025E-5	0.0	0.3504103323865334	0.0013211161544196054
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGTT	30	0.005740813	29.338285	30
GTATCAA	2115	0.0	22.986273	1
TATACCG	70	3.218234E-5	21.998175	5
TATACTG	220	0.0	20.998259	5
CGTGACG	55	0.0044831103	20.000856	10
TACGACG	375	0.0	18.771776	5
GATATAC	460	0.0	18.650627	1
TGTAGGA	1045	0.0	18.524199	2
CGACGGT	370	0.0	18.430904	7
CGTAAAT	60	0.0074179936	18.331238	16
GTAGGAC	980	0.0	17.95713	3
TAGGACG	785	0.0	17.934818	4
CGCAATA	370	0.0	17.839725	36
GTACCGT	75	0.001291831	17.59854	6
AACGCAG	2735	0.0	17.534195	6
TACGAAT	390	0.0	17.486279	41
GCGAAAG	390	0.0	17.48573	18
AGGACGT	840	0.0	17.28428	5
TATGGCG	945	0.0	16.992764	16
CTGTAGG	1030	0.0	16.872389	1
>>END_MODULE