FastQCFastQC Report
Thu 2 Feb 2017
SRR4064247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646052
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98221.5203110585525623No Hit
GTACTTTTTTTTTTTTTTTTTTTTT67411.0434144619937713No Hit
TATCAACGCAGAGTACTTTTTTTTT62940.9742249849857286No Hit
GTACATGGGGTGGTATCAACGCAAA48630.7527257867787732No Hit
GAGTACTTTTTTTTTTTTTTTTTTT45240.7002532303901233No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36540.565589147622792No Hit
GGTATCAACGCAGAGTACTTTTTTT31590.4889699281172413No Hit
GTATCAACGCAGAGTACATGGGGTG31470.4871124924928643No Hit
GTACATGGGTGGTATCAACGCAAAA23860.36932011664695724No Hit
TATCAACGCAGAGTACATGGGGTGG19540.3024524341693857No Hit
GCAGAGTACTTTTTTTTTTTTTTTT18020.27892491626061056No Hit
GTACATGGGAGTGGTATCAACGCAA16260.2516825271030815No Hit
GGTATCAACGCAGAGTACATGGGGT14450.2236662064353953No Hit
ACGCAGAGTACATGGGGTGGTATCA12820.19843603920427455No Hit
ATCAACGCAGAGTACTTTTTTTTTT11830.18311219530316444No Hit
GTATCAACGCAGAGTACATGGGAGT10820.1674787787979915No Hit
GTATCAACGCAGAGTACATGGGTGG10200.15788202807204374No Hit
GAGTACATGGGGTGGTATCAACGCA7580.11732801693981289No Hit
GTATCAACGCAGAGTACATGGGAAG7240.11206528267074477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC350.002167525516.2866448
GTGTTAG350.002170892216.2828621
CCCTTAA456.7637407E-414.7751881
GACTAGC400.005280817414.2475041
TCTAGGA551.9602725E-413.8157612
TAGGACG707.2425555E-613.5722044
TGTAGGA1051.36788E-913.5690512
ACGGTAT801.9947001E-613.0622359
CGAGCCG905.384845E-712.66738915
CTATTCC751.4745505E-512.6673894
AGGCCCG905.38992E-712.66640910
GGCTATA604.092318E-412.6644471
TTAGAGT550.00306379612.09164
TACTCAG550.00306379612.09165
GGGTAGG1103.8073267E-812.0887911
AGGACGT802.8586132E-511.8756785
TCGGCGT802.8608803E-511.87475913
CGGTCGG802.8608803E-511.87475910
GTATTAG658.0240733E-411.6902581
ACTAGCA658.0240733E-411.6902582
Produced by FastQC (version 0.11.5)