##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064247_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646052
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.942113018766292	32.0	32.0	32.0	32.0	32.0
2	30.64086791775275	32.0	32.0	32.0	32.0	32.0
3	30.684791626680205	32.0	32.0	32.0	32.0	32.0
4	30.634419520410123	32.0	32.0	32.0	32.0	32.0
5	30.635326568140027	32.0	32.0	32.0	32.0	32.0
6	34.20334276497867	36.0	36.0	36.0	32.0	36.0
7	34.06198107892244	36.0	36.0	36.0	32.0	36.0
8	33.95151319088866	36.0	36.0	36.0	32.0	36.0
9	34.04219784165981	36.0	36.0	36.0	32.0	36.0
10	33.82751698005733	36.0	36.0	36.0	32.0	36.0
11	34.192682632357766	36.0	36.0	36.0	32.0	36.0
12	33.98525660473151	36.0	36.0	36.0	32.0	36.0
13	34.071063939125644	36.0	36.0	36.0	32.0	36.0
14	33.9726074681295	36.0	36.0	36.0	32.0	36.0
15	33.94733086500777	36.0	36.0	36.0	32.0	36.0
16	33.924832676007505	36.0	36.0	36.0	32.0	36.0
17	33.81109724913784	36.0	36.0	36.0	32.0	36.0
18	33.82383616179503	36.0	36.0	36.0	32.0	36.0
19	33.78927547627745	36.0	36.0	36.0	32.0	36.0
20	33.73602744051563	36.0	36.0	36.0	32.0	36.0
21	33.689605480673386	36.0	36.0	36.0	27.0	36.0
22	33.72962857478964	36.0	36.0	36.0	32.0	36.0
23	33.742618241256125	36.0	36.0	36.0	32.0	36.0
24	33.72951712865218	36.0	36.0	36.0	27.0	36.0
25	33.382819030047116	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6.0
5	7.0
6	17.0
7	17.0
8	30.0
9	51.0
10	61.0
11	28.0
12	47.0
13	28.0
14	181.0
15	256.0
16	340.0
17	462.0
18	584.0
19	868.0
20	1272.0
21	1896.0
22	2612.0
23	3990.0
24	5455.0
25	7086.0
26	9442.0
27	11740.0
28	15160.0
29	19603.0
30	25119.0
31	32515.0
32	44700.0
33	62105.0
34	128173.0
35	272201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.91649246035064	17.181078561628773	11.934139173038746	22.968289804981836
2	16.033899616887894	20.98231492589296	38.733330753453814	24.250454703765335
3	18.777977970540707	28.302716223662785	27.85259208351134	25.066713722285172
4	12.091872036704235	15.640319552218243	35.049819744934055	37.21798866614347
5	13.39790465693711	38.759976718410414	31.37194196939957	16.470176655252907
6	31.021239047648535	35.76380073686492	18.561565373540976	14.653394841945572
7	28.58702114219894	32.291240912074706	20.97220674023706	18.149531205489293
8	29.5589888423341	30.08048507820895	19.809424712851907	20.55110136660504
9	29.585511084268312	13.491757454683423	19.52395332450618	37.398778136542084
10	17.369722819782524	26.521455641408064	29.98942202109091	26.1193995177185
11	35.088586030664395	22.83877961901812	22.98513241443395	19.087501935883537
12	26.6024310610801	24.6154227407054	28.37848721586509	20.403658982349405
13	29.824436407567745	20.786202730645023	26.55974350832901	22.82961735345822
14	24.93095687650923	19.753226013807076	27.511303246072327	27.80451386361137
15	24.24810778244648	26.817063179235245	26.04499814921222	22.88983088910606
16	23.195306231819856	26.906610989092687	26.01863009624827	23.879452682839183
17	21.623183018531854	26.074390725553513	26.622060110432205	25.680366145482424
18	22.234666468384905	25.490050283016235	29.483259132579242	22.79202411601962
19	24.085508481140113	24.372705444969405	29.453489272713192	22.08829680117729
20	25.94932394750214	22.840164086802414	28.34137862657859	22.869133339116857
21	27.19921929457689	22.69389841535387	27.496321080595443	22.610561209473797
22	25.584680948626154	23.485762707138242	27.809853405688102	23.119702938547505
23	23.402907199214255	24.47277386092353	28.920327001817192	23.20399193804502
24	23.19400057936979	25.40118259597942	28.74169866884369	22.663118155807098
25	23.556768027909587	25.307158802378883	28.686486561850806	22.449586607860724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	463.0
1	463.0
2	1035.5
3	1608.0
4	1608.0
5	1608.0
6	4656.5
7	7705.0
8	7705.0
9	7705.0
10	6932.5
11	6160.0
12	6160.0
13	6160.0
14	4286.0
15	2412.0
16	2412.0
17	2412.0
18	4275.0
19	6138.0
20	6138.0
21	6138.0
22	9215.5
23	12293.0
24	12293.0
25	12293.0
26	18090.5
27	23888.0
28	23888.0
29	23888.0
30	28701.5
31	33515.0
32	33515.0
33	33515.0
34	35910.5
35	38306.0
36	38306.0
37	38306.0
38	42893.5
39	47481.0
40	47481.0
41	47481.0
42	56127.5
43	64774.0
44	64774.0
45	64774.0
46	77010.0
47	89246.0
48	89246.0
49	89246.0
50	91189.5
51	93133.0
52	93133.0
53	93133.0
54	79778.5
55	66424.0
56	66424.0
57	66424.0
58	60933.5
59	55443.0
60	55443.0
61	55443.0
62	48397.0
63	41351.0
64	41351.0
65	41351.0
66	33897.0
67	26443.0
68	26443.0
69	26443.0
70	20287.0
71	14131.0
72	14131.0
73	14131.0
74	10870.5
75	7610.0
76	7610.0
77	7610.0
78	5912.5
79	4215.0
80	4215.0
81	4215.0
82	2899.5
83	1584.0
84	1584.0
85	1584.0
86	1212.5
87	841.0
88	841.0
89	841.0
90	606.5
91	372.0
92	372.0
93	372.0
94	249.0
95	126.0
96	126.0
97	126.0
98	258.0
99	390.0
100	390.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0411731563403565
2	0.004179230154848217
3	0.0012382904162513235
4	0.0035600849467225548
5	0.007429742497507941
6	0.011144613746261911
7	0.02167008228439816
8	0.03374341384284856
9	0.04906725774395869
10	0.05742571805365512
11	0.054484778315058234
12	0.0631528112288175
13	0.06454588794710023
14	0.06826075919585421
15	0.05897358107396928
16	0.06655810987350863
17	0.06299802492678608
18	0.07832186882789621
19	0.07770272361977054
20	0.08358460309696433
21	0.07460699757914224
22	0.08188195377461875
23	0.0852872524193099
24	0.08095323596243027
25	0.08342981679493292
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	646052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.50838639468456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.85871587734846	31.092987193055105
2	15.887171609211382	14.142247454028853
3	6.164847388996709	8.231622289611831
4	2.8917758344113813	5.148331048191724
5	1.6047725444865657	3.5712918242794585
6	0.8838212124674875	2.3602473616992916
7	0.5612809814300967	1.7487197558234961
8	0.38531072167806635	1.371964678596971
9	0.27210805133302113	1.0899981260851286
>10	1.273819335945894	10.015360012756243
>50	0.11640283828607996	3.6633492195037496
>100	0.09018597879882861	7.816005630422536
>500	0.003845138630957785	1.0377362811258264
>1k	0.00489381280303718	5.153986474491043
>5k	0.0010486741720793957	3.556152650328788
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9822	1.5203110585525623	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6741	1.0434144619937713	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6294	0.9742249849857286	No Hit
GTACATGGGGTGGTATCAACGCAAA	4863	0.7527257867787732	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4524	0.7002532303901233	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3654	0.565589147622792	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3159	0.4889699281172413	No Hit
GTATCAACGCAGAGTACATGGGGTG	3147	0.4871124924928643	No Hit
GTACATGGGTGGTATCAACGCAAAA	2386	0.36932011664695724	No Hit
TATCAACGCAGAGTACATGGGGTGG	1954	0.3024524341693857	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1802	0.27892491626061056	No Hit
GTACATGGGAGTGGTATCAACGCAA	1626	0.2516825271030815	No Hit
GGTATCAACGCAGAGTACATGGGGT	1445	0.2236662064353953	No Hit
ACGCAGAGTACATGGGGTGGTATCA	1282	0.19843603920427455	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1183	0.18311219530316444	No Hit
GTATCAACGCAGAGTACATGGGAGT	1082	0.1674787787979915	No Hit
GTATCAACGCAGAGTACATGGGTGG	1020	0.15788202807204374	No Hit
GAGTACATGGGGTGGTATCAACGCA	758	0.11732801693981289	No Hit
GTATCAACGCAGAGTACATGGGAAG	724	0.11206528267074477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.5478630203141544E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGACC	35	0.0021675255	16.286644	8
GTGTTAG	35	0.0021708922	16.282862	1
CCCTTAA	45	6.7637407E-4	14.775188	1
GACTAGC	40	0.0052808174	14.247504	1
TCTAGGA	55	1.9602725E-4	13.815761	2
TAGGACG	70	7.2425555E-6	13.572204	4
TGTAGGA	105	1.36788E-9	13.569051	2
ACGGTAT	80	1.9947001E-6	13.062235	9
CGAGCCG	90	5.384845E-7	12.667389	15
CTATTCC	75	1.4745505E-5	12.667389	4
AGGCCCG	90	5.38992E-7	12.666409	10
GGCTATA	60	4.092318E-4	12.664447	1
TTAGAGT	55	0.003063796	12.0916	4
TACTCAG	55	0.003063796	12.0916	5
GGGTAGG	110	3.8073267E-8	12.088791	1
AGGACGT	80	2.8586132E-5	11.875678	5
TCGGCGT	80	2.8608803E-5	11.874759	13
CGGTCGG	80	2.8608803E-5	11.874759	10
GTATTAG	65	8.0240733E-4	11.690258	1
ACTAGCA	65	8.0240733E-4	11.690258	2
>>END_MODULE