FastQCFastQC Report
Thu 2 Feb 2017
SRR4064247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646052
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32460.5024363363939746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.35477020425600414No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA22560.3491978973828732No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA16690.2583383380904324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.21948697628054706No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13120.20307962826521703No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA12530.19394723644536352No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11960.18512441722957287No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA9710.15029749927250438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.14983314036641013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9560.14797570474203314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7680.11887587996012705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTA207.852965E-444.00108333
CGTCGTA1450.027.31313310
TACCGTC1500.026.3986057
ATACCGT1600.024.7486956
CGGATAC450.001397309424.44504725
TAGGACG1102.0190782E-1023.9987344
CCGTCGT1750.023.8881519
ACCGTCG1850.023.7825288
TCGCACG1254.0017767E-1122.88056422
CCGATCG1254.0017767E-1122.87879418
GAGTAAT703.2136755E-521.9988381
GTATAGC602.8717984E-421.9988381
GTCTTAC602.8717984E-421.9988381
TCTAGAT1351.1277734E-1021.1840672
AGGACGT1259.949872E-1021.1188855
GTGTAAG755.4698052E-520.532251
AGATACC2150.020.4640374
CCGTGAC654.9305E-420.3066229
GTCGTAG1850.020.21827911
TCGTACA550.004480752620.00049235
Produced by FastQC (version 0.11.5)