##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646052
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.768591382736993	32.0	32.0	32.0	32.0	32.0
2	31.334569043977883	32.0	32.0	32.0	32.0	32.0
3	31.43541541547739	32.0	32.0	32.0	32.0	32.0
4	31.542347984372775	32.0	32.0	32.0	32.0	32.0
5	31.474553131946035	32.0	32.0	32.0	32.0	32.0
6	34.9798003875849	36.0	36.0	36.0	36.0	36.0
7	35.017144130813	36.0	36.0	36.0	36.0	36.0
8	34.99598948691437	36.0	36.0	36.0	36.0	36.0
9	35.0759830478042	36.0	36.0	36.0	36.0	36.0
10	34.92322908991846	36.0	36.0	36.0	32.0	36.0
11	35.08588317968213	36.0	36.0	36.0	36.0	36.0
12	35.004496542074016	36.0	36.0	36.0	36.0	36.0
13	35.04211270919369	36.0	36.0	36.0	36.0	36.0
14	34.975785230910205	36.0	36.0	36.0	36.0	36.0
15	34.960761672435034	36.0	36.0	36.0	36.0	36.0
16	34.95905283166061	36.0	36.0	36.0	36.0	36.0
17	34.9245045290472	36.0	36.0	36.0	36.0	36.0
18	34.88932005473244	36.0	36.0	36.0	32.0	36.0
19	34.8930891011869	36.0	36.0	36.0	32.0	36.0
20	34.877872059834196	36.0	36.0	36.0	32.0	36.0
21	34.87252728882505	36.0	36.0	36.0	32.0	36.0
22	34.87953446471801	36.0	36.0	36.0	32.0	36.0
23	34.84104994644394	36.0	36.0	36.0	32.0	36.0
24	34.82669661265657	36.0	36.0	36.0	32.0	36.0
25	34.79557527877013	36.0	36.0	36.0	32.0	36.0
26	34.7317816522509	36.0	36.0	36.0	32.0	36.0
27	34.73382173571168	36.0	36.0	36.0	32.0	36.0
28	34.70836403261657	36.0	36.0	36.0	32.0	36.0
29	34.66142044293648	36.0	36.0	36.0	32.0	36.0
30	34.61465021391467	36.0	36.0	36.0	32.0	36.0
31	34.58139747264926	36.0	36.0	36.0	32.0	36.0
32	34.48829505984038	36.0	36.0	36.0	32.0	36.0
33	34.378960207537475	36.0	36.0	36.0	32.0	36.0
34	34.300023837090514	36.0	36.0	36.0	32.0	36.0
35	34.186333298248435	36.0	36.0	36.0	32.0	36.0
36	34.1432794883384	36.0	36.0	36.0	32.0	36.0
37	34.10785509525549	36.0	36.0	36.0	32.0	36.0
38	34.03784060725762	36.0	36.0	36.0	32.0	36.0
39	33.9846003108109	36.0	36.0	36.0	32.0	36.0
40	33.91307046491614	36.0	36.0	36.0	32.0	36.0
41	33.834555113210705	36.0	36.0	36.0	32.0	36.0
42	33.72270188777374	36.0	36.0	36.0	27.0	36.0
43	33.63917145988248	36.0	36.0	36.0	27.0	36.0
44	33.5179366366794	36.0	36.0	36.0	21.0	36.0
45	33.348208812912894	36.0	36.0	36.0	21.0	36.0
46	33.21581389733334	36.0	36.0	36.0	14.0	36.0
47	33.04302130478661	36.0	36.0	36.0	14.0	36.0
48	32.87750521629838	36.0	36.0	36.0	14.0	36.0
49	32.76170029657055	36.0	36.0	36.0	14.0	36.0
50	32.20641836880004	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	14.0
21	52.0
22	192.0
23	510.0
24	1227.0
25	2465.0
26	4781.0
27	8307.0
28	13286.0
29	17639.0
30	22862.0
31	29124.0
32	38807.0
33	56235.0
34	103298.0
35	347246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.582664720297096	18.28947551738497	12.575910735322118	23.551949026995818
2	15.255855998093018	21.787424134776572	38.056519107742936	24.900200759387474
3	18.276885261380293	28.190344105960673	28.524528249317775	25.008242383341255
4	11.721657080235028	16.194052491130744	35.338022326376205	36.74626810225802
5	13.005764241887649	38.7145307188895	31.68042200937386	16.59928302984899
6	31.20099063539974	35.95201609782524	17.999071279312748	14.84792198746227
7	28.088760657036893	32.20127791570957	21.375369165330348	18.334592261923188
8	29.326741500684157	30.34631887216509	19.858772978026536	20.46816664912422
9	29.09710903386286	13.195324798350585	19.831064922521115	37.87650124526544
10	17.200088538175898	26.686056320805392	30.251420557883378	25.862434583135336
11	35.12163107613628	22.5142867756775	23.09303275897296	19.271049389213253
12	26.17239196286361	24.809651250443075	28.69030463539256	20.32765215130075
13	29.84753502050925	20.53401439517065	26.87470010061141	22.74375048370869
14	24.6643066878621	19.584986324609048	27.944852650951706	27.805854336577145
15	24.362830310469782	26.61662701367293	26.28026023921823	22.740282436639056
16	23.229759055912773	26.429396231204784	26.413762572711992	23.92708214017045
17	21.598759231082376	25.624447605529593	27.341804284801047	25.434988878586985
18	22.24791694721901	24.35550354695556	30.550563658681178	22.846015847144248
19	23.912935800833367	23.64747729284949	30.603883278745364	21.835703627571775
20	25.44474593796388	22.15069275574536	29.681770408471266	22.722790897819493
21	26.720449747079183	22.268640914353643	28.780810213419354	22.23009912514782
22	25.215926891333822	22.856209716864896	29.218855448168256	22.70900794363302
23	23.127466914325517	23.602662332636793	30.210510022444083	23.059360730593607
24	23.115703118953643	24.26112529989939	29.988855351752957	22.63431622939401
25	23.371891257675596	24.350621709767648	29.95568392718109	22.321803105375665
26	22.853410070695173	24.99144754052937	30.174470173200852	21.98067221557461
27	22.876771066656968	24.818539679787744	29.71893580429994	22.58575344925535
28	22.023243790459148	25.114934166328702	30.206556433062655	22.6552656101495
29	21.813813888188026	24.97062834446025	30.137253475452418	23.078304291899304
30	21.88919262811483	25.61027333434465	29.848673884171127	22.65186015336939
31	22.454899799692264	25.671588189583378	28.82078494626985	23.05272706445451
32	22.307151724543594	25.740295282233223	28.74450873494256	23.208044258280626
33	22.330215811747514	25.40687216923158	28.985520706596944	23.27739131242396
34	22.98059538846373	25.34564851276717	28.902747851499615	22.771008247269485
35	23.24262073299661	25.685684787000046	28.669433295203184	22.402261184800157
36	22.54532113727378	26.705221818542075	28.13664375132546	22.61281329285868
37	23.160760833895743	26.871590705046838	27.880060926151806	22.08758753490561
38	22.398468120940425	27.053238060445445	28.267317230233868	22.28097658838026
39	22.68596416547347	27.04415463797841	27.860376920397815	22.409504276150304
40	22.76426036199053	27.472799629743715	27.87556169209599	21.887378316169766
41	22.09219709951101	27.780486634500928	27.884661524482645	22.24265474150541
42	22.99491977202438	28.089388484197425	27.14067680648387	21.77501493729432
43	22.321424425309154	27.53500927956808	27.17543569081315	22.968130604309618
44	21.916188241339974	27.923683098493445	26.7282518346302	23.431876825536378
45	22.44375787482625	27.99166003659182	26.456939598728262	23.107642489853664
46	22.52694862888596	27.957228919392485	26.138157772535614	23.37766467918594
47	22.75662656570222	27.854639679522503	26.01464933842696	23.374084416348314
48	23.330407822413747	28.669852223001325	24.877523253168143	23.122216701416782
49	22.74529835152078	28.35322343471868	25.04852565590899	23.85295255785156
50	22.39560276266868	29.03820494642763	25.229783822104444	23.33640846879924
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	143.0
1	98.5
2	54.0
3	186.0
4	318.0
5	410.5
6	503.0
7	532.5
8	562.0
9	816.5
10	1071.0
11	1646.5
12	2222.0
13	3819.5
14	5417.0
15	7695.0
16	9973.0
17	11333.5
18	12694.0
19	12681.5
20	12669.0
21	12455.5
22	12242.0
23	12239.0
24	12236.0
25	9638.0
26	7040.0
27	6775.0
28	6510.0
29	7376.5
30	8243.0
31	10318.5
32	12394.0
33	14827.5
34	17261.0
35	18409.5
36	19558.0
37	20717.0
38	21876.0
39	23222.5
40	24569.0
41	26611.5
42	28654.0
43	29780.0
44	30906.0
45	34007.0
46	37108.0
47	39270.0
48	41432.0
49	42916.0
50	44400.0
51	43068.0
52	41736.0
53	40928.0
54	40120.0
55	40074.0
56	40028.0
57	39199.0
58	38370.0
59	35095.5
60	31821.0
61	27765.0
62	23709.0
63	20961.0
64	18213.0
65	15791.0
66	13369.0
67	11434.5
68	9500.0
69	8456.5
70	7413.0
71	5812.5
72	4212.0
73	3567.5
74	2923.0
75	2322.0
76	1721.0
77	1431.0
78	1141.0
79	941.0
80	741.0
81	591.5
82	442.0
83	364.0
84	286.0
85	194.0
86	102.0
87	83.0
88	64.0
89	45.5
90	27.0
91	23.0
92	19.0
93	16.5
94	14.0
95	15.0
96	16.0
97	11.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009751537027979172
2	7.739315101570771E-4
3	1.5478630203141544E-4
4	0.0
5	0.0
6	3.095726040628309E-4
7	0.0
8	0.0
9	7.739315101570771E-4
10	4.643589060942463E-4
11	0.0
12	1.5478630203141544E-4
13	3.095726040628309E-4
14	1.5478630203141544E-4
15	0.004798375362973879
16	0.0015478630203141542
17	1.5478630203141544E-4
18	7.739315101570771E-4
19	0.0
20	0.0013930767182827389
21	0.0
22	0.0
23	3.095726040628309E-4
24	3.095726040628309E-4
25	0.0017026493223455698
26	0.006036665779225202
27	0.006655810987350863
28	0.004643589060942463
29	0.003250512342659724
30	0.004643589060942463
31	0.0071201698934451095
32	0.0040244438528168015
33	0.0040244438528168015
34	0.0030957260406283085
35	0.0029409397385968933
36	0.007894101403602187
37	0.0037148712487539703
38	0.007429742497507941
39	0.004179230154848217
40	0.0020122219264084007
41	0.0035600849467225548
42	0.0027861534365654778
43	0.0013930767182827389
44	0.0013930767182827389
45	9.287178121884926E-4
46	0.0012382904162513235
47	0.002476580832502647
48	0.0017026493223455698
49	3.095726040628309E-4
50	0.0015478630203141542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	646052.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.167759312763096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69451069650644	37.196152266779244
2	14.675340167770553	15.01808547074816
3	5.5621338756692955	8.53805782246838
4	2.6091019165515306	5.340075955543105
5	1.3586914223596627	3.4760597839807477
6	0.7803340797667497	2.3956767826230916
7	0.5106841234661541	1.8291393620055896
8	0.33092753276333703	1.354625627712076
9	0.23088461421595427	1.0632463532299896
>10	1.0733127849606716	9.750665433605086
>50	0.09677808172426865	3.471325091518636
>100	0.07151836810106414	7.095410196565472
>500	0.0033476682940905876	1.1914184881796779
>1k	0.0024346678502476997	2.2800613650407584
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3246	0.5024363363939746	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2292	0.35477020425600414	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	2256	0.3491978973828732	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1669	0.2583383380904324	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	0.21948697628054706	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1312	0.20307962826521703	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	1253	0.19394723644536352	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1196	0.18512441722957287	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	971	0.15029749927250438	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	968	0.14983314036641013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	956	0.14797570474203314	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	768	0.11887587996012705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.5478630203141544E-4	0.0
14	0.0	0.0	0.0	1.5478630203141544E-4	0.0
15	0.0	0.0	0.0	1.5478630203141544E-4	0.0
16	0.0	0.0	0.0	1.5478630203141544E-4	0.0
17	0.0	0.0	0.0	1.5478630203141544E-4	0.0
18	0.0	0.0	0.0	4.643589060942463E-4	0.0
19	0.0	0.0	0.0	4.643589060942463E-4	0.0
20	0.0	0.0	0.0	9.287178121884926E-4	0.0
21	0.0	0.0	0.0	0.0013930767182827389	0.0
22	0.0	0.0	0.0	0.002631367134534062	0.0
23	0.0	0.0	0.0	0.006810597289382279	0.0
24	0.0	0.0	0.0	0.012073331558450404	0.0
25	0.0	0.0	0.0	0.015788202807204374	0.0
26	0.0	0.0	0.0	0.020896150774241083	0.0
27	0.0	0.0	0.0	0.02646845764737204	0.0
28	0.0	0.0	0.0	0.04024443852816801	0.0
29	0.0	0.0	0.0	0.0682607591958542	0.0
30	0.0	0.0	0.0	0.12228117860481819	0.0
31	0.0	0.0	0.0	0.2117476611789763	0.0
32	0.0	0.0	0.0	0.2931652560475008	0.0
33	0.0	0.0	0.0	0.36452174128398335	0.0
34	0.0	0.0	0.0	0.43618779912452865	0.0
35	0.0	0.0	0.0	0.5261186406047811	0.0
36	0.0	0.0	0.0	0.6377195643694316	0.0
37	0.0	0.0	0.0	0.7895649266622501	0.0
38	0.0	0.0	0.0	0.983666949409645	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGTA	20	7.852965E-4	44.001083	33
CGTCGTA	145	0.0	27.313133	10
TACCGTC	150	0.0	26.398605	7
ATACCGT	160	0.0	24.748695	6
CGGATAC	45	0.0013973094	24.445047	25
TAGGACG	110	2.0190782E-10	23.998734	4
CCGTCGT	175	0.0	23.888151	9
ACCGTCG	185	0.0	23.782528	8
TCGCACG	125	4.0017767E-11	22.880564	22
CCGATCG	125	4.0017767E-11	22.878794	18
GAGTAAT	70	3.2136755E-5	21.998838	1
GTATAGC	60	2.8717984E-4	21.998838	1
GTCTTAC	60	2.8717984E-4	21.998838	1
TCTAGAT	135	1.1277734E-10	21.184067	2
AGGACGT	125	9.949872E-10	21.118885	5
GTGTAAG	75	5.4698052E-5	20.53225	1
AGATACC	215	0.0	20.464037	4
CCGTGAC	65	4.9305E-4	20.30662	29
GTCGTAG	185	0.0	20.218279	11
TCGTACA	55	0.0044807526	20.000492	35
>>END_MODULE