FastQCFastQC Report
Thu 2 Feb 2017
SRR4064246_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064246_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267012
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35940.2836595075658321No Hit
TATCAACGCAGAGTACTTTTTTTTT23570.1860282301982933No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19530.15414218649862826No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13630.10757593456099863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCG504.5585202E-617.1009613
GACTAAT801.2906094E-714.2474241
AGAACCG1400.013.5716535
AGCGTAT1550.013.485168
CGGTTCT2400.013.45908912
CACGCGC655.4480628E-513.15458412
TAGGACC4550.013.1525064
GGTCATA655.4601885E-513.1514671
AACCGCG1102.741217E-912.9552737
TAGAACC1650.012.6653774
GTATTAG1457.2759576E-1212.4460261
GCGCGCT701.0900213E-412.21497215
TATGCGC550.003065709312.09206610
CGTATAT1650.012.09206510
ATAGCGT1650.012.0915876
AATAGCG1650.012.0911095
TAGTACT951.0405347E-611.9987784
CGCGGTT2800.011.87613610
AATCGAA802.8637314E-511.8756677
TCGAACG802.8753278E-511.8709783
Produced by FastQC (version 0.11.5)