##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1267012
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29566176168813	32.0	32.0	32.0	32.0	32.0
2	31.39599467092656	32.0	32.0	32.0	32.0	32.0
3	31.47357325739614	32.0	32.0	32.0	32.0	32.0
4	31.55989998516194	32.0	32.0	32.0	32.0	32.0
5	31.507588720548817	32.0	32.0	32.0	32.0	32.0
6	35.090416665351235	36.0	36.0	36.0	36.0	36.0
7	35.109583808203865	36.0	36.0	36.0	36.0	36.0
8	35.05468614346194	36.0	36.0	36.0	36.0	36.0
9	35.154989060877085	36.0	36.0	36.0	36.0	36.0
10	35.019879054026326	36.0	36.0	36.0	36.0	36.0
11	35.15924158571505	36.0	36.0	36.0	36.0	36.0
12	35.06581310989951	36.0	36.0	36.0	36.0	36.0
13	35.10682377120343	36.0	36.0	36.0	36.0	36.0
14	35.05086928932007	36.0	36.0	36.0	36.0	36.0
15	35.0266437886934	36.0	36.0	36.0	36.0	36.0
16	35.0362411721436	36.0	36.0	36.0	36.0	36.0
17	34.995749842937556	36.0	36.0	36.0	36.0	36.0
18	34.996368621607374	36.0	36.0	36.0	36.0	36.0
19	34.98960783323283	36.0	36.0	36.0	36.0	36.0
20	34.98303488838306	36.0	36.0	36.0	36.0	36.0
21	34.97186056643505	36.0	36.0	36.0	36.0	36.0
22	34.96009587912348	36.0	36.0	36.0	36.0	36.0
23	34.90832525658794	36.0	36.0	36.0	32.0	36.0
24	34.878844083560374	36.0	36.0	36.0	32.0	36.0
25	34.84942999750594	36.0	36.0	36.0	32.0	36.0
26	34.792110887663256	36.0	36.0	36.0	32.0	36.0
27	34.7808150199051	36.0	36.0	36.0	32.0	36.0
28	34.75578210782534	36.0	36.0	36.0	32.0	36.0
29	34.73169314892045	36.0	36.0	36.0	32.0	36.0
30	34.70993487038797	36.0	36.0	36.0	32.0	36.0
31	34.68902346623394	36.0	36.0	36.0	32.0	36.0
32	34.650483973316746	36.0	36.0	36.0	32.0	36.0
33	34.63275485946463	36.0	36.0	36.0	32.0	36.0
34	34.62145346689692	36.0	36.0	36.0	32.0	36.0
35	34.57390774515159	36.0	36.0	36.0	32.0	36.0
36	34.54038162227351	36.0	36.0	36.0	32.0	36.0
37	34.533657929048815	36.0	36.0	36.0	32.0	36.0
38	34.4816023841921	36.0	36.0	36.0	32.0	36.0
39	34.47255906021411	36.0	36.0	36.0	32.0	36.0
40	34.45806432772539	36.0	36.0	36.0	32.0	36.0
41	34.42899751541422	36.0	36.0	36.0	32.0	36.0
42	34.37588041786503	36.0	36.0	36.0	32.0	36.0
43	34.36390815556601	36.0	36.0	36.0	32.0	36.0
44	34.29713372880446	36.0	36.0	36.0	32.0	36.0
45	34.2629604139503	36.0	36.0	36.0	32.0	36.0
46	34.22473031036802	36.0	36.0	36.0	32.0	36.0
47	34.19374165359128	36.0	36.0	36.0	32.0	36.0
48	34.132080832699295	36.0	36.0	36.0	32.0	36.0
49	34.11313783926277	36.0	36.0	36.0	32.0	36.0
50	33.52811338803421	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	10.0
21	52.0
22	147.0
23	397.0
24	1072.0
25	2555.0
26	5196.0
27	9283.0
28	15500.0
29	23231.0
30	33181.0
31	46904.0
32	66438.0
33	106505.0
34	217710.0
35	738827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57844584386451	18.02539163326424	11.931415436736408	26.46474708613485
2	15.582550056945227	20.67565530419327	37.49521510141617	26.246579537445335
3	18.257120915669763	24.649271866690555	28.92304462406285	28.170562593576832
4	12.113855275246012	16.092902040391095	36.55261355062146	35.24062913374143
5	13.930649433470244	37.317878599413426	33.51388937121354	15.237582595902802
6	33.53743622771127	36.31902876698489	16.800525332121026	13.343009673182808
7	29.575962974304897	30.691422022837983	21.13989449192273	18.592720510934388
8	27.708814123307434	33.42738663880058	19.61449457463702	19.249304663254964
9	27.647443243704007	13.724589501466461	18.544809928365993	40.08315732646354
10	15.531207403998923	26.87509224611698	32.11064640553879	25.4830539443453
11	37.326639368845754	20.89490865122035	22.922198053372817	18.856253926561077
12	24.740669181246254	23.441311874956096	29.380013275338573	22.438005668459073
13	29.675810276991804	19.538754779973246	25.472354094893074	25.31308084814188
14	23.385608637658738	19.74183313470296	25.452285301615614	31.420272926022687
15	24.93573163329658	27.72881077672179	22.43587567968188	24.89958191029975
16	25.268151847741844	25.69017037014821	24.456711333647984	24.584966448461966
17	23.651078284972833	25.81909247899783	25.62943365966542	24.900395576363916
18	24.387868417459412	24.924803098956833	26.90663903198641	23.780689451597347
19	25.540326374177987	24.59297938772482	25.630222918172834	24.23647131992436
20	25.797965750673047	24.19007689800261	25.372631319736417	24.639326031587927
21	26.717584363841855	24.049022424412712	24.68832181542085	24.54507139632458
22	25.692497600646565	24.498266782845885	25.22296623225741	24.58626938425014
23	24.32253461320503	24.244002771897755	25.69790734347696	25.735555271420253
24	24.74530547557711	24.85264497146032	25.660927160680913	24.74112239228166
25	24.756805307701963	24.463037958498457	25.871968896336117	24.908187837463466
26	24.243890426269154	25.43399639763652	26.215876604054163	24.106236572040167
27	24.858614361544685	24.893028395524595	25.46054423111076	24.78781301181996
28	24.217728848448875	24.891392886234048	25.925528354665374	24.965349910651703
29	24.299120743816	24.74798339358159	25.701352823248985	25.251543039353425
30	24.133968240437518	25.266998011771513	25.880594211168965	24.718439536622
31	25.04583919067288	24.716933624536182	25.069755290154383	25.167471894636556
32	24.481418192294182	25.2425284044942	25.08119798100184	25.194855422209766
33	24.2716615139085	24.683434901997618	25.617166774931157	25.427736809162727
34	24.90796926204924	24.891315329195372	25.801361991778798	24.399353416976595
35	25.356734968353805	24.736123860180573	25.692336956393053	24.214804215072572
36	24.372172249287242	25.309493664891214	25.354011498861794	24.964322586959746
37	25.09270236266711	25.025217884097184	25.289551824133085	24.592527929102623
38	24.62091343858012	24.972867418564764	25.511809283407803	24.894409859447308
39	25.317335909591034	24.54903650803423	25.12024903824448	25.013378544130255
40	25.556342202448185	24.970382349853708	25.437713746299274	24.035561701398837
41	24.463243570051823	25.032479269307206	26.126040086443002	24.378237074197965
42	25.54816885044961	25.598524377836938	25.25905866734649	23.59424810436696
43	24.556982206675336	24.76179802240267	25.732838092111077	24.948381678810915
44	24.17205998421468	25.39865824782952	25.13954222573007	25.289739542225732
45	24.444689994830288	25.522279093445515	25.199468032628385	24.833562879095812
46	24.38782798805056	24.976854866397527	25.220973871452756	25.414343274099156
47	24.70206876629358	24.996704780712037	25.617468845378838	24.683757607615547
48	25.205112278007146	25.36509788167064	24.656330875800816	24.773458964521396
49	24.561191655005363	25.501120353335526	24.97428988382895	24.963398107830162
50	24.64269816163587	25.957698366845673	24.5888700516813	24.81073341983716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	66.5
2	47.0
3	145.0
4	243.0
5	279.5
6	316.0
7	277.5
8	239.0
9	353.0
10	467.0
11	705.0
12	943.0
13	1601.5
14	2260.0
15	2992.0
16	3724.0
17	3840.0
18	3956.0
19	3962.5
20	3969.0
21	4424.5
22	4880.0
23	5512.5
24	6145.0
25	6993.0
26	7841.0
27	10345.5
28	12850.0
29	15032.0
30	17214.0
31	19793.0
32	22372.0
33	26369.5
34	30367.0
35	35465.5
36	40564.0
37	45733.5
38	50903.0
39	54197.0
40	57491.0
41	61396.5
42	65302.0
43	66295.0
44	67288.0
45	73864.5
46	80441.0
47	85272.0
48	90103.0
49	93503.5
50	96904.0
51	94005.5
52	91107.0
53	90291.0
54	89475.0
55	89530.0
56	89585.0
57	86399.0
58	83213.0
59	76075.0
60	68937.0
61	60967.5
62	52998.0
63	46229.5
64	39461.0
65	33644.0
66	27827.0
67	23656.5
68	19486.0
69	17194.0
70	14902.0
71	11605.5
72	8309.0
73	7021.0
74	5733.0
75	4429.5
76	3126.0
77	2722.5
78	2319.0
79	1932.5
80	1546.0
81	1251.0
82	956.0
83	762.5
84	569.0
85	429.5
86	290.0
87	190.5
88	91.0
89	73.5
90	56.0
91	46.5
92	37.0
93	27.5
94	18.0
95	24.0
96	30.0
97	20.0
98	10.0
99	13.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01089176740236083
2	3.946292537087257E-4
3	3.946292537087257E-4
4	0.0
5	0.0
6	3.157034029669806E-4
7	0.0
8	0.0
9	0.0012628136118679224
10	0.0016574428655766482
11	0.0
12	7.892585074174515E-5
13	5.524809551922161E-4
14	1.578517014834903E-4
15	0.004656625193762963
16	4.7355510445047086E-4
17	0.0
18	3.946292537087257E-4
19	0.0
20	7.103326566757063E-4
21	0.0
22	3.157034029669806E-4
23	7.892585074174514E-4
24	3.157034029669806E-4
25	0.0018152945670601385
26	0.00520910614895518
27	0.006866549014531828
28	0.004735551044504709
29	0.002525627223735845
30	0.004340921790795983
31	0.006550845611564848
32	0.0037095149848620215
33	0.004183070089312493
34	0.0034727374326367863
35	0.00276240477596108
36	0.008208288477141496
37	0.004261995940054238
38	0.006708697313048337
39	0.004577699343021218
40	0.0024467013729940995
41	0.003946292537087257
42	0.0016574428655766482
43	0.0015785170148349028
44	9.471102089009417E-4
45	0.0013417394626096674
46	5.524809551922161E-4
47	0.0019731462685436286
48	0.0018152945670601385
49	7.892585074174515E-5
50	0.0012628136118679224
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1267012.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.58362808116405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97231720059098	41.24456017421853
2	13.520861641672518	14.489936430885148
3	4.071075683807589	6.544290159942494
4	1.6789771842739676	3.598627559955853
5	0.9100901490595664	2.438296603376948
6	0.5593825872808244	1.798424910716099
7	0.3869621657974523	1.4514385741500695
8	0.28490933768203225	1.2213180789763836
9	0.21219413454921265	1.0233118428020544
>10	1.2386625033017458	12.150331471140298
>50	0.08618473332065048	3.1725139812876937
>100	0.07038143201083097	7.661355966719209
>500	0.006371363075375954	2.240303475778653
>1k	0.0016298835774217555	0.9652907700505673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	1.578517014834903E-4
15	0.0	0.0	0.0	0.0	1.578517014834903E-4
16	0.0	0.0	0.0	0.0	1.578517014834903E-4
17	0.0	0.0	0.0	0.0	1.578517014834903E-4
18	0.0	0.0	0.0	0.0	1.578517014834903E-4
19	0.0	0.0	0.0	0.0	1.578517014834903E-4
20	0.0	0.0	0.0	2.3677755222523543E-4	1.578517014834903E-4
21	0.0	0.0	0.0	4.7355510445047086E-4	1.578517014834903E-4
22	0.0	0.0	0.0	7.892585074174514E-4	1.578517014834903E-4
23	0.0	0.0	0.0	0.0015785170148349028	2.3677755222523543E-4
24	0.0	0.0	0.0	0.0022099238207688643	2.3677755222523543E-4
25	0.0	0.0	0.0	0.0031570340296698056	2.3677755222523543E-4
26	0.0	0.0	0.0	0.003709514984862022	3.157034029669806E-4
27	0.0	0.0	0.0	0.004656625193762963	3.157034029669806E-4
28	0.0	0.0	0.0	0.007892585074174514	3.157034029669806E-4
29	0.0	0.0	0.0	0.01689013205873346	3.157034029669806E-4
30	0.0	0.0	0.0	0.030623230087797118	3.157034029669806E-4
31	0.0	0.0	0.0	0.057773722742957445	3.157034029669806E-4
32	0.0	0.0	0.0	0.08981761814410598	3.946292537087257E-4
33	0.0	0.0	0.0	0.11752059175445852	3.946292537087257E-4
34	0.0	0.0	0.0	0.14940663545412355	7.103326566757063E-4
35	0.0	0.0	0.0	0.18594930434755155	7.103326566757063E-4
36	0.0	0.0	0.0	0.23448870255372484	7.892585074174514E-4
37	0.0	0.0	0.0	0.3172029941310737	7.892585074174514E-4
38	0.0	0.0	0.0	0.42075371030424336	7.892585074174514E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	70	3.213196E-5	22.002052	10
AATCGGT	70	3.2171138E-5	21.998579	19
AGTATCG	85	6.1151422E-6	20.705362	8
GTATCAA	2645	0.0	20.628712	1
CAATCGG	75	5.475623E-5	20.53201	18
TAGGACG	515	0.0	19.649992	4
GTCCTAC	595	0.0	19.227926	1
GATATAC	485	0.0	19.052572	1
TCCTACA	590	0.0	19.015722	2
GCGACTC	60	0.007411681	18.333595	31
GTATATG	145	6.3027983E-9	18.207878	1
TTAGGAC	385	0.0	17.713142	3
TTAATAC	125	4.8562106E-7	17.598864	3
ATACACT	645	0.0	17.394913	4
TTAACGG	90	2.2152337E-4	17.111357	35
GTAGGAC	630	0.0	17.110006	3
CTAGATA	245	0.0	17.060123	3
ACTGTTC	595	0.0	17.007977	8
AACGCAG	3225	0.0	16.98562	6
ATAATAC	130	7.3224146E-7	16.921984	3
>>END_MODULE