FastQCFastQC Report
Thu 2 Feb 2017
SRR4064245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826840
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39610.21682249129644635No Hit
TATCAACGCAGAGTACTTTTTTTTT27960.1530511703269033No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19100.10455212279126798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAC759.655723E-713.93584415
CATACGG500.001501091913.2983895
CGACCAT1800.013.19501510
CGTTATT1301.4551915E-1113.1482922
GCGTTAT1457.2759576E-1212.4450651
CGCATCG2150.012.37364613
CGAACGA1209.997166E-911.8764916
CCGTCGT1701.8189894E-1211.7358029
ATCGCCA2350.011.72487416
CCGACCA2050.011.5858679
TCGCCAG2550.011.55015217
TCGCGTA1001.9289928E-611.4004939
ATTAGAC1001.9359977E-611.39705853
GGTTAGG752.0791702E-411.39705851
TCTTATA1751.8189894E-1211.3951862
CAAGACG3100.011.3364054
GTATTAG2950.011.2682781
GCGTAAC1104.966623E-711.22775811
GTATTAC855.3378208E-511.1735871
CCGGCAC600.00588762211.0804743
Produced by FastQC (version 0.11.5)