##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064245_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1826840
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30509568435112	32.0	32.0	32.0	32.0	32.0
2	31.03488209148037	32.0	32.0	32.0	32.0	32.0
3	31.04940991000854	32.0	32.0	32.0	32.0	32.0
4	31.068059600183926	32.0	32.0	32.0	32.0	32.0
5	31.01610923780955	32.0	32.0	32.0	32.0	32.0
6	34.6225783319831	36.0	36.0	36.0	32.0	36.0
7	34.59044306014758	36.0	36.0	36.0	32.0	36.0
8	34.56271102012218	36.0	36.0	36.0	32.0	36.0
9	34.66800540824593	36.0	36.0	36.0	32.0	36.0
10	34.460712487136256	36.0	36.0	36.0	32.0	36.0
11	34.628286002058196	36.0	36.0	36.0	32.0	36.0
12	34.50714840927503	36.0	36.0	36.0	32.0	36.0
13	34.5752266208316	36.0	36.0	36.0	32.0	36.0
14	34.495026384357686	36.0	36.0	36.0	32.0	36.0
15	34.44362779444286	36.0	36.0	36.0	32.0	36.0
16	34.43329574565917	36.0	36.0	36.0	32.0	36.0
17	34.378950537540234	36.0	36.0	36.0	32.0	36.0
18	34.36956438440148	36.0	36.0	36.0	32.0	36.0
19	34.379000897725035	36.0	36.0	36.0	32.0	36.0
20	34.35818243524337	36.0	36.0	36.0	32.0	36.0
21	34.335999868625606	36.0	36.0	36.0	32.0	36.0
22	34.32186234152964	36.0	36.0	36.0	32.0	36.0
23	34.2786762934904	36.0	36.0	36.0	32.0	36.0
24	34.25884094939896	36.0	36.0	36.0	32.0	36.0
25	33.85094972739813	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	17.0
5	20.0
6	75.0
7	18.0
8	88.0
9	113.0
10	161.0
11	45.0
12	110.0
13	104.0
14	182.0
15	295.0
16	521.0
17	814.0
18	928.0
19	1230.0
20	1626.0
21	2247.0
22	3385.0
23	4862.0
24	7213.0
25	10789.0
26	15571.0
27	20907.0
28	29744.0
29	41283.0
30	56261.0
31	79322.0
32	116029.0
33	170587.0
34	388025.0
35	874263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.67057667781753	17.249243908142027	11.089566288296744	25.9906131257437
2	16.76475563338685	19.562576673347046	37.66238600613222	26.01028168713388
3	18.55353004095673	23.933794830978222	28.922666117803423	28.590009010261625
4	12.330410125030108	15.303871335041276	36.446385951082796	35.919332588845826
5	14.233894719784876	36.84867748271717	34.188807161454626	14.728620636043333
6	34.06023982275256	35.949324400155696	16.836481351407727	13.153954425684017
7	30.025361005592345	30.667708409757637	21.057737899044362	18.24919268560566
8	27.90622541012557	33.37690264367331	19.436274818490105	19.28059712771101
9	27.308274712004753	13.922129699860623	18.486515497115256	40.28308009101937
10	15.282731379447837	26.952698824426342	32.51662803587625	25.247941760249574
11	37.118227275465664	21.05685446549354	22.698245786986075	19.12667247205472
12	24.186342282596016	23.872277332386865	29.390596783655038	22.550783601362077
13	29.31962236784737	19.646358750544998	25.501125052856672	25.532893828750964
14	23.277909998931882	19.653711428015853	25.60185578247688	31.466522790575386
15	25.010885752453333	27.492563456405776	22.379628224852816	25.116922566288068
16	25.48278219260019	25.933247446961875	23.975936479140785	24.608033881297146
17	23.789857898403714	26.010770450930476	25.545971597207924	24.653400053457894
18	24.41217056051344	25.299002403194326	26.307719529090047	23.981107507202186
19	25.15677724703098	25.146917042815197	25.66167448179149	24.03463122836233
20	25.42939814688008	24.67994403608511	25.316221318219718	24.574436498815093
21	26.32038511597741	24.46130653706763	24.75382464689668	24.464483700058285
22	25.4435973580546	24.672290414593917	25.09974127253594	24.784370954815547
23	24.30852331400429	24.487935554027736	25.56561420616247	25.637926925805505
24	24.466430096618303	25.245818483797073	25.398650477065885	24.889100942518738
25	24.623947191826783	24.75958312220107	25.60730768704036	25.009161998931784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	108.0
2	380.5
3	653.0
4	653.0
5	653.0
6	1877.0
7	3101.0
8	3101.0
9	3101.0
10	3435.0
11	3769.0
12	3769.0
13	3769.0
14	4694.5
15	5620.0
16	5620.0
17	5620.0
18	9424.5
19	13229.0
20	13229.0
21	13229.0
22	20541.0
23	27853.0
24	27853.0
25	27853.0
26	40752.0
27	53651.0
28	53651.0
29	53651.0
30	67621.0
31	81591.0
32	81591.0
33	81591.0
34	99425.0
35	117259.0
36	117259.0
37	117259.0
38	135104.5
39	152950.0
40	152950.0
41	152950.0
42	174594.0
43	196238.0
44	196238.0
45	196238.0
46	218363.0
47	240488.0
48	240488.0
49	240488.0
50	244716.0
51	248944.0
52	248944.0
53	248944.0
54	231001.0
55	213058.0
56	213058.0
57	213058.0
58	194310.5
59	175563.0
60	175563.0
61	175563.0
62	152555.5
63	129548.0
64	129548.0
65	129548.0
66	105332.5
67	81117.0
68	81117.0
69	81117.0
70	60514.5
71	39912.0
72	39912.0
73	39912.0
74	30507.0
75	21102.0
76	21102.0
77	21102.0
78	16659.0
79	12216.0
80	12216.0
81	12216.0
82	8366.0
83	4516.0
84	4516.0
85	4516.0
86	3407.0
87	2298.0
88	2298.0
89	2298.0
90	1625.0
91	952.0
92	952.0
93	952.0
94	613.0
95	274.0
96	274.0
97	274.0
98	552.0
99	830.0
100	830.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036839569967813277
2	0.005309715136519892
3	0.0018611372643471786
4	0.004379146504346303
5	0.00941516498434455
6	0.013192178844343238
7	0.022662083159992116
8	0.03454051805303146
9	0.04680212826520111
10	0.05517724595476342
11	0.05200236473911234
12	0.060377482428674654
13	0.06284075233736944
14	0.0654135009086729
15	0.05796895185128418
16	0.06415449628867334
17	0.06043222175997898
18	0.07406231525475684
19	0.07225591732171399
20	0.07543079853736506
21	0.07181800267127937
22	0.07482866589301745
23	0.0785509404217118
24	0.0714895666834534
25	0.0747191872304088
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1826840.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.551122958248506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36241057791575	40.129304276683065
2	13.771329907853408	14.473977025724189
3	4.340668472523284	6.843210078616914
4	1.7889755718356364	3.7605070097934985
5	0.9694967264711265	2.547407084020179
6	0.5771208601277474	1.8197009569406033
7	0.39585349376470674	1.4561781936996954
8	0.28152512780603106	1.1835569285737089
9	0.2094064855475451	0.9904091373237356
>10	1.1324467723190326	10.700593976570026
>50	0.0860272777188607	3.152145744706214
>100	0.07408183776436549	8.044491359842826
>500	0.00773146802040629	2.673656029126476
>1k	0.002925420332045623	2.224862198378985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3961	0.21682249129644635	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2796	0.1530511703269033	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1910	0.10455212279126798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.7369665652164394E-4
13	0.0	0.0	0.0	0.0	2.7369665652164394E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAC	75	9.655723E-7	13.935844	15
CATACGG	50	0.0015010919	13.298389	5
CGACCAT	180	0.0	13.195015	10
CGTTATT	130	1.4551915E-11	13.148292	2
GCGTTAT	145	7.2759576E-12	12.445065	1
CGCATCG	215	0.0	12.373646	13
CGAACGA	120	9.997166E-9	11.87649	16
CCGTCGT	170	1.8189894E-12	11.735802	9
ATCGCCA	235	0.0	11.724874	16
CCGACCA	205	0.0	11.585867	9
TCGCCAG	255	0.0	11.550152	17
TCGCGTA	100	1.9289928E-6	11.400493	9
ATTAGAC	100	1.9359977E-6	11.3970585	3
GGTTAGG	75	2.0791702E-4	11.3970585	1
TCTTATA	175	1.8189894E-12	11.395186	2
CAAGACG	310	0.0	11.336405	4
GTATTAG	295	0.0	11.268278	1
GCGTAAC	110	4.966623E-7	11.227758	11
GTATTAC	85	5.3378208E-5	11.173587	1
CCGGCAC	60	0.005887622	11.080474	3
>>END_MODULE