FastQCFastQC Report
Thu 2 Feb 2017
SRR4064245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826840
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG752.0654643E-623.466365
GTATCAA26450.022.2088641
TAACCCG1658.185452E-1118.66795528
ACCGTCG3600.017.110428
AACCCGT1753.6252459E-916.34398729
AACGCAG36000.016.2553446
CGAGCCG6250.016.19134315
CGTCGTA3950.016.15168810
TAGACAG4250.016.046855
GGTATCA13300.015.714941
TACCGTC4400.015.4993737
GTACATG56800.015.22246551
CCGTCGT4050.015.2096799
TCAACGC38250.015.1265994
GTATTAC1603.824698E-715.125631
TACATGG56450.015.1207342
ACCCGTT1901.0757503E-815.05408330
ACGAGAC3400.014.88134319
CGAACGA3550.014.87223116
TATCAAC39450.014.8333662
Produced by FastQC (version 0.11.5)