FastQCFastQC Report
Thu 2 Feb 2017
SRR4064244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929563
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26220.28206802551306365No Hit
TATCAACGCAGAGTACTTTTTTTTT18280.1966515448657057No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14170.15243722050038566No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10280.11058959962907303No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9710.10445768603096292No Hit
GAATAGGACCGCGGTTCTATTTTGT9700.10435010859941715No Hit
GCGCAAGACGGACCAGAGCGAAAGC9380.10090763078995184No Hit
CTATTGGAGCTGGAATTACCGCGGC9320.1002621662006771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC607.67177E-817.41735810
TCTAACC350.002174207516.2802243
CGCGTAA551.1269096E-515.54607110
GCGTAAC551.1269096E-515.54607111
GTAAGAC508.733006E-515.1948753
AACCGAC456.745644E-414.78154817
CGGACAT1058.913048E-1114.4728695
GCGCGCT400.00526806214.25363615
TCGCGTA602.563803E-514.2513339
TCTTATA759.697451E-713.9286372
AAGACCG551.9626337E-413.815015
GCTCGTA852.6919952E-713.4130189
CGTCTTA500.001495876713.30339215
AACCGTC500.001499523513.2990967
GTATTAG2000.013.2948011
CTATTAT500.001503177913.2948011
GGTCGCG655.451356E-513.1529527
CCGATAA801.9948347E-613.0637239
AAGCTCG905.401562E-712.6658067
AGACCGT604.0971965E-412.6637596
Produced by FastQC (version 0.11.5)