FastQCFastQC Report
Thu 2 Feb 2017
SRR4064244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929563
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10110.10876078329279458No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9920.10671681209342455No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9410.1012303630845892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTT250.002353346435.1974646
TACGTTC300.00574636429.3312177
CGGATCG500.002577850622.00314926
CGCAATA2600.021.15573536
ATACGAA2950.020.13522340
CTCTATA550.00448611319.9974821
GCTATAT1152.0228254E-719.1280251
TCTAGCG3000.019.06734328
CTAGCGG3000.019.06734329
CGGTCCG1759.094947E-1218.85781339
GTAATAC1051.793016E-618.8557823
GATATAC2000.018.6976451
TAGGACA1801.4551915E-1118.3320124
TACCGCT600.00741392918.332017
CGAATGC3200.017.87467243
TTAGGAC2100.017.8082393
TAGCGGC3250.017.60062430
ATACACT2500.017.598734
GTATCAA20700.017.5340251
GCGCAAT3150.017.46187635
Produced by FastQC (version 0.11.5)