##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064244_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	929563
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285156573572742	32.0	32.0	32.0	32.0	32.0
2	31.35160715303858	32.0	32.0	32.0	32.0	32.0
3	31.45069349791246	32.0	32.0	32.0	32.0	32.0
4	31.54099829704926	32.0	32.0	32.0	32.0	32.0
5	31.476724009023595	32.0	32.0	32.0	32.0	32.0
6	35.06587504020707	36.0	36.0	36.0	36.0	36.0
7	35.08766807628961	36.0	36.0	36.0	36.0	36.0
8	35.02365950451986	36.0	36.0	36.0	36.0	36.0
9	35.144147303625466	36.0	36.0	36.0	36.0	36.0
10	34.97844363426685	36.0	36.0	36.0	36.0	36.0
11	35.14727995843208	36.0	36.0	36.0	36.0	36.0
12	35.03941314359543	36.0	36.0	36.0	36.0	36.0
13	35.09159034944377	36.0	36.0	36.0	36.0	36.0
14	35.03036910892538	36.0	36.0	36.0	36.0	36.0
15	35.00210098723809	36.0	36.0	36.0	36.0	36.0
16	35.02197269039323	36.0	36.0	36.0	36.0	36.0
17	34.97884167076357	36.0	36.0	36.0	36.0	36.0
18	34.972760318558294	36.0	36.0	36.0	36.0	36.0
19	34.9736381503997	36.0	36.0	36.0	36.0	36.0
20	34.96401642492225	36.0	36.0	36.0	36.0	36.0
21	34.96245762793915	36.0	36.0	36.0	36.0	36.0
22	34.940377360114375	36.0	36.0	36.0	36.0	36.0
23	34.89319605018702	36.0	36.0	36.0	32.0	36.0
24	34.85350643259252	36.0	36.0	36.0	32.0	36.0
25	34.8367888997303	36.0	36.0	36.0	32.0	36.0
26	34.77211980253087	36.0	36.0	36.0	32.0	36.0
27	34.77038780588298	36.0	36.0	36.0	32.0	36.0
28	34.74210354758096	36.0	36.0	36.0	32.0	36.0
29	34.716886321852314	36.0	36.0	36.0	32.0	36.0
30	34.697734311714214	36.0	36.0	36.0	32.0	36.0
31	34.68584055088251	36.0	36.0	36.0	32.0	36.0
32	34.65128237677274	36.0	36.0	36.0	32.0	36.0
33	34.627909028220785	36.0	36.0	36.0	32.0	36.0
34	34.606536619895586	36.0	36.0	36.0	32.0	36.0
35	34.567415011139644	36.0	36.0	36.0	32.0	36.0
36	34.536693048238796	36.0	36.0	36.0	32.0	36.0
37	34.53960301776211	36.0	36.0	36.0	32.0	36.0
38	34.47738345867897	36.0	36.0	36.0	32.0	36.0
39	34.47776858588391	36.0	36.0	36.0	32.0	36.0
40	34.45830137387138	36.0	36.0	36.0	32.0	36.0
41	34.44571696592915	36.0	36.0	36.0	32.0	36.0
42	34.368057893870564	36.0	36.0	36.0	32.0	36.0
43	34.362842539989224	36.0	36.0	36.0	32.0	36.0
44	34.2967340567557	36.0	36.0	36.0	32.0	36.0
45	34.26102480412839	36.0	36.0	36.0	32.0	36.0
46	34.231674453479755	36.0	36.0	36.0	32.0	36.0
47	34.209979312859915	36.0	36.0	36.0	32.0	36.0
48	34.156315386907615	36.0	36.0	36.0	32.0	36.0
49	34.13286780992789	36.0	36.0	36.0	32.0	36.0
50	33.52591809269517	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	3.0
21	18.0
22	89.0
23	264.0
24	720.0
25	1621.0
26	3302.0
27	6405.0
28	10586.0
29	16493.0
30	24590.0
31	34700.0
32	51134.0
33	82455.0
34	170139.0
35	527039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04081417479217	17.77884647453794	11.777217608536377	26.40312174213351
2	15.828490529907763	20.369788652241173	37.05008186686576	26.751638950985306
3	18.094367227505487	24.529885106932312	28.83729936744266	28.53844829811954
4	11.956048164567653	15.721150691238787	36.125684864823576	36.197116279369986
5	14.06865376526389	37.25438727660202	33.48024824568103	15.196710712453058
6	34.601784286957574	35.75362080298292	16.526546459127392	13.11804845093211
7	30.11436556747633	30.56823475116802	20.82903471846448	18.488364962891165
8	28.22616648898461	33.23217468853644	19.216771751887716	19.324887070591235
9	27.60574168444403	13.518648210483963	18.25694715632137	40.61866294875063
10	15.439662461782747	26.95357128257207	31.94994588802802	25.656820367617154
11	37.753264440672055	20.714917348170427	22.47918912347966	19.052629087677854
12	24.72255690864495	23.386548474547098	29.129265458926806	22.761629157881146
13	29.72580457766934	19.193356828421308	25.42837655827083	25.652462035638518
14	23.58194119172127	19.40427921507203	25.34760957568234	31.666170017524365
15	25.237811424845997	27.18903775935485	22.266701666885417	25.30644914891374
16	25.653297875430447	25.500428700676565	23.88642713218074	24.959846291712253
17	23.966745664360563	25.855159897715378	25.28865714319524	24.889437294728815
18	24.9126736441689	24.86254234534871	26.26600355652519	23.9587804539572
19	25.859168081311413	24.67516959600328	25.18670083329568	24.278961489389626
20	25.802616956752335	24.215617614057507	25.060001957930318	24.92176347125984
21	26.96976966596132	24.11079184520038	24.398238742290733	24.52119974654757
22	25.946277982234662	24.209978237085597	24.91686953977299	24.92687424090675
23	24.51670370952091	24.040238652084764	25.452851582586916	25.99020605580741
24	24.959739059620617	25.071619829145153	25.148537858193276	24.82010325304095
25	25.084073300299288	24.334890365834504	25.253941171281465	25.327095162584744
26	24.63862913602348	25.29305574083717	25.719944573304815	24.348370549834538
27	25.252553506916577	24.718506389415452	24.93894606382315	25.08999403984482
28	24.6790327196208	24.73250037115372	25.640697381698786	24.9477695275267
29	24.584336967034197	24.618224732905237	25.47467077766506	25.322767522395512
30	24.582476267525024	25.063795818953277	25.589869266982813	24.76385864653889
31	25.138516821052402	24.73625321412818	24.815866765645676	25.309363199173738
32	24.88661009363812	24.944703594051227	24.703723147893143	25.464963164417515
33	24.631748586379324	24.453163325903038	25.22409222745307	25.690995860264564
34	25.17608304385105	24.702081582902384	25.387694681092547	24.734140692154018
35	25.688217492757165	24.59638595045501	25.427765806491177	24.28763075029665
36	24.692169568440296	25.209658210048747	24.842252033106362	25.255920188404595
37	25.515878437620692	25.08802826371579	24.911272458906698	24.484820839756825
38	25.009413805049242	24.850078430444352	25.108178068308725	25.032329696197685
39	25.482910014943183	24.614183003934283	24.71219047229702	25.19071650882551
40	25.78625080416293	24.969608558230004	24.930341739659916	24.31379889794715
41	24.55275543960625	25.079691240149536	25.691616685941742	24.675936634302467
42	25.956382914633412	25.475394386925203	24.865525461946934	23.70269723649445
43	25.092302528529874	24.413158166514624	25.283362307864433	25.211176997091073
44	24.677963840723617	25.05126114244035	24.874186975689415	25.396588041146618
45	24.883277105530404	25.223656126822387	24.89263645282889	25.000430314818324
46	24.664517975209723	25.061803566856504	24.81394383136932	25.45973462656445
47	25.039616071053967	24.941826502586746	25.314265981171474	24.704291445187817
48	25.787559343294436	25.332069638071797	24.210988339506578	24.66938267912719
49	24.87647394845524	25.485686254048957	24.553633381779143	25.084206415716665
50	25.100936262502056	25.540074896696996	24.22341204909488	25.135576791706065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	31.5
2	28.0
3	69.5
4	111.0
5	141.5
6	172.0
7	162.5
8	153.0
9	206.5
10	260.0
11	398.5
12	537.0
13	970.5
14	1404.0
15	1866.5
16	2329.0
17	2455.5
18	2582.0
19	2684.5
20	2787.0
21	3143.5
22	3500.0
23	4093.5
24	4687.0
25	5298.0
26	5909.0
27	7400.0
28	8891.0
29	10269.5
30	11648.0
31	12945.0
32	14242.0
33	16596.5
34	18951.0
35	21931.5
36	24912.0
37	28933.5
38	32955.0
39	35652.0
40	38349.0
41	42268.5
42	46188.0
43	47655.0
44	49122.0
45	54583.0
46	60044.0
47	64435.5
48	68827.0
49	70971.0
50	73115.0
51	71382.0
52	69649.0
53	68466.5
54	67284.0
55	68134.0
56	68984.0
57	67364.5
58	65745.0
59	59912.0
60	54079.0
61	47145.0
62	40211.0
63	34857.5
64	29504.0
65	25055.5
66	20607.0
67	17208.5
68	13810.0
69	12536.0
70	11262.0
71	8619.5
72	5977.0
73	5110.0
74	4243.0
75	3239.5
76	2236.0
77	1946.5
78	1657.0
79	1363.5
80	1070.0
81	895.0
82	720.0
83	566.0
84	412.0
85	297.0
86	182.0
87	129.0
88	76.0
89	56.5
90	37.0
91	30.0
92	23.0
93	18.5
94	14.0
95	19.0
96	24.0
97	16.0
98	8.0
99	9.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008821349386754851
2	5.378871577289544E-4
3	3.227322946373726E-4
4	0.0
5	0.0
6	4.3030972618316355E-4
7	0.0
8	0.0
9	0.0010757743154579088
10	9.681968839121179E-4
11	1.0757743154579089E-4
12	3.227322946373726E-4
13	7.530420208205361E-4
14	0.0
15	0.004840984419560589
16	0.0010757743154579088
17	0.0
18	4.3030972618316355E-4
19	1.0757743154579089E-4
20	0.0010757743154579088
21	0.0
22	0.0
23	9.681968839121179E-4
24	2.1515486309158177E-4
25	0.001828816336278445
26	0.005056139282652171
27	0.00613191359811008
28	0.0031197455148279356
29	0.002581858357098981
30	0.0037652101041026806
31	0.007853152502842733
32	0.0029045906517363536
33	0.0037652101041026806
34	0.0032273229463737263
35	0.002581858357098981
36	0.007960729934388525
37	0.0034424778094653084
38	0.008283462229025897
39	0.004518252124923217
40	0.002689435788644772
41	0.004087942398740053
42	0.0020439711993700265
43	0.0011833517470036996
44	9.681968839121179E-4
45	0.0011833517470036996
46	5.378871577289544E-4
47	0.0017212389047326542
48	0.0015060840416410723
49	2.1515486309158177E-4
50	0.0017212389047326542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	929563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.34664561896412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10626214200214	41.36120058015375
2	13.800259811947917	14.999956588991317
3	4.349464181823029	7.091363655657415
4	1.925976339576405	4.186814143898743
5	1.0340965772602524	2.809984011007334
6	0.649171040747683	2.116816109856506
7	0.4005392598649922	1.523757564865544
8	0.29901195938227176	1.3000237593904331
9	0.21554237648724403	1.0542604635739437
>10	1.0617756703593466	10.30767822914235
>50	0.07835696163884583	2.9556758560296985
>100	0.07278246628360278	7.742803698319133
>500	0.006562353431467854	2.440403230863379
>1k	1.9885919489296528E-4	0.10926210825042627
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1011	0.10876078329279458	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	992	0.10671681209342455	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	941	0.1012303630845892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	1.0757743154579087E-4	0.0
19	0.0	0.0	0.0	2.1515486309158175E-4	0.0
20	0.0	0.0	0.0	3.227322946373726E-4	0.0
21	0.0	0.0	0.0	6.454645892747452E-4	0.0
22	0.0	0.0	0.0	6.454645892747452E-4	0.0
23	0.0	0.0	0.0	0.0011833517470036996	0.0
24	0.0	0.0	0.0	0.0020439711993700265	0.0
25	0.0	0.0	0.0	0.002259126062461608	0.0
26	0.0	0.0	0.0	0.0024742809255531902	1.0757743154579087E-4
27	0.0	0.0	0.0	0.0029045906517363536	1.0757743154579087E-4
28	0.0	0.0	0.0	0.004195519830285844	1.0757743154579087E-4
29	0.0	0.0	0.0	0.010542588291487505	1.0757743154579087E-4
30	0.0	0.0	0.0	0.01731996647887233	1.0757743154579087E-4
31	0.0	0.0	0.0	0.03334900377919517	1.0757743154579087E-4
32	0.0	0.0	0.0	0.04862499905869747	1.0757743154579087E-4
33	0.0	0.0	0.0	0.06400857176974557	1.0757743154579087E-4
34	0.0	0.0	0.0	0.07949972191233945	1.0757743154579087E-4
35	0.0	0.0	0.0	0.09929396931676497	1.0757743154579087E-4
36	0.0	0.0	0.0	0.1310293116227733	1.0757743154579087E-4
37	0.0	0.0	0.0	0.17126327102089905	1.0757743154579087E-4
38	0.0	0.0	0.0	0.230968745528813	1.0757743154579087E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGTT	25	0.0023533464	35.197464	6
TACGTTC	30	0.005746364	29.331217	7
CGGATCG	50	0.0025778506	22.003149	26
CGCAATA	260	0.0	21.155735	36
ATACGAA	295	0.0	20.135223	40
CTCTATA	55	0.004486113	19.997482	1
GCTATAT	115	2.0228254E-7	19.128025	1
TCTAGCG	300	0.0	19.067343	28
CTAGCGG	300	0.0	19.067343	29
CGGTCCG	175	9.094947E-12	18.857813	39
GTAATAC	105	1.793016E-6	18.855782	3
GATATAC	200	0.0	18.697645	1
TAGGACA	180	1.4551915E-11	18.332012	4
TACCGCT	60	0.007413929	18.33201	7
CGAATGC	320	0.0	17.874672	43
TTAGGAC	210	0.0	17.808239	3
TAGCGGC	325	0.0	17.600624	30
ATACACT	250	0.0	17.59873	4
GTATCAA	2070	0.0	17.534025	1
GCGCAAT	315	0.0	17.461876	35
>>END_MODULE