FastQCFastQC Report
Thu 2 Feb 2017
SRR4064243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699739
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59380.8486021216482146No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41410.5917920824764662No Hit
TATCAACGCAGAGTACTTTTTTTTT40540.5793588752377672No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28500.40729471988841554No Hit
GTACATGGGGTGGTATCAACGCAAA27440.39214621451712717No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22480.3212626422137397No Hit
GGTATCAACGCAGAGTACTTTTTTT19260.27524548438775026No Hit
GTATCAACGCAGAGTACATGGGGTG17840.254952203607345No Hit
GTACATGGGTGGTATCAACGCAAAA12800.18292534787970943No Hit
TATCAACGCAGAGTACATGGGGTGG11690.16706229036826586No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11380.16263206709930417No Hit
GATTAAGAGGGACGGCCGGGGGCAT10620.15177087456894642No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10520.15034177028863618No Hit
GTACATGGGAGTGGTATCAACGCAA9870.14105259246661972No Hit
GAATAGGACCGCGGTTCTATTTTGT9860.14090968203858867No Hit
GTATCTGATCGTCTTCGAACCTCCG9720.13890893604615434No Hit
ATCAGATACCGTCGTAGTTCCGACC9490.13562199620144083No Hit
CTATTGGAGCTGGAATTACCGCGGC9300.1329066980688514No Hit
GAACTACGACGGTATCTGATCGTCT9190.13133468336051013No Hit
GTCCTATTCCATTATTCCTAGCTGC8950.1279048330877656No Hit
GAATAACGCCGCCGCATCGCCAGTC8780.1254753558112382No Hit
GCGCAAGACGGACCAGAGCGAAAGC8740.1249037140991141No Hit
GGTATCAACGCAGAGTACATGGGGT8590.12276005767864877No Hit
CTTTAATATACGCTATTGGAGCTGG8240.11775819269756295No Hit
CTCTTAATCATGGCCTCAGTTCCGA8060.11518580499300453No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8020.11461416328088045No Hit
GTACATGGGGAATAATTGCAATCCC7930.11332796942860124No Hit
GCTTTGAACACTCTAATTTTTTCAA7890.11275632771647714No Hit
ATATTAAAGTTGCTGCAGTTAAAAA7710.11018394001191872No Hit
GAATAATGGAATAGGACCGCGGTTC7700.1100410295838877No Hit
TCGTAGTTCCGACCATAAACGATGC7650.1093264774437326No Hit
ATCAACGCAGAGTACTTTTTTTTTT7530.10761155230736032No Hit
GTCCTGTATTGTTATTTTTCGTCAC7040.10060894133384019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGTT607.653034E-817.41948717
TATATCT402.764021E-416.6205584
GTCCTAA508.696315E-515.2013731
TTCGCAG707.2314233E-613.57459615
TCGCAGT707.2367693E-613.57362616
GCATCGC1550.013.48605314
CGACCAT1205.0931703E-1113.45762510
TAGACAG852.6982707E-713.409145
GGACCGT500.001498704413.2992996
CGGTCCA1800.013.19374910
CGCATCG1600.013.06274613
CCGACCA1251.0186341E-1012.9193199
AAGACAA604.0937777E-412.6641885
TTAGAGT751.4798918E-512.6632814
GCCGGTC1900.012.4993418
CGCCGGT1900.012.4993417
GGTCCAA2000.012.34934911
GTTTTCG1405.2750693E-1112.21713715
CGCCCGA701.08760054E-412.21626316
TATTAGC701.09196684E-412.2110222
Produced by FastQC (version 0.11.5)