##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064243_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 699739 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.025502365882137 32.0 32.0 32.0 32.0 32.0 2 30.68997440474234 32.0 32.0 32.0 32.0 32.0 3 30.73285753688161 32.0 32.0 32.0 32.0 32.0 4 30.722992430034626 32.0 32.0 32.0 32.0 32.0 5 30.690833296414805 32.0 32.0 32.0 32.0 32.0 6 34.278123700408294 36.0 36.0 36.0 32.0 36.0 7 34.15164225518372 36.0 36.0 36.0 32.0 36.0 8 34.09115255831103 36.0 36.0 36.0 32.0 36.0 9 34.17683879274987 36.0 36.0 36.0 32.0 36.0 10 33.94018484034761 36.0 36.0 36.0 32.0 36.0 11 34.2458316600904 36.0 36.0 36.0 32.0 36.0 12 34.04412788196742 36.0 36.0 36.0 32.0 36.0 13 34.13932337628744 36.0 36.0 36.0 32.0 36.0 14 34.03537318914624 36.0 36.0 36.0 32.0 36.0 15 33.98138448764468 36.0 36.0 36.0 32.0 36.0 16 33.98339523736708 36.0 36.0 36.0 32.0 36.0 17 33.889983265188874 36.0 36.0 36.0 32.0 36.0 18 33.89687154781997 36.0 36.0 36.0 32.0 36.0 19 33.893701794526244 36.0 36.0 36.0 32.0 36.0 20 33.839976048212264 36.0 36.0 36.0 32.0 36.0 21 33.80503873587152 36.0 36.0 36.0 32.0 36.0 22 33.817560547575596 36.0 36.0 36.0 32.0 36.0 23 33.783803675370386 36.0 36.0 36.0 32.0 36.0 24 33.778504556699 36.0 36.0 36.0 32.0 36.0 25 33.403346104761916 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 11.0 5 7.0 6 36.0 7 11.0 8 30.0 9 43.0 10 66.0 11 24.0 12 46.0 13 40.0 14 189.0 15 216.0 16 301.0 17 456.0 18 548.0 19 853.0 20 1262.0 21 1912.0 22 2682.0 23 3849.0 24 5421.0 25 7278.0 26 9854.0 27 12375.0 28 15798.0 29 20613.0 30 26166.0 31 34572.0 32 47602.0 33 65299.0 34 138496.0 35 303679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.423909515796275 17.473339271442605 11.842381801363096 24.260369411398024 2 16.521626036710597 20.68276048358628 37.475506975241856 25.320106504461272 3 18.293148269095823 26.57530644356822 28.433904370456226 26.697640916879728 4 12.12779063236469 15.474905924908569 35.649064537013345 36.74823890571339 5 14.248431403375877 37.60258407534981 32.076693298269184 16.07229122300513 6 32.44977359526151 35.83426715759142 17.732750931918495 13.983208315228577 7 29.41345477623973 31.388331417920263 21.167458561324665 18.030755244515344 8 29.31602377705154 30.377070413353575 19.930292867170458 20.376612942424423 9 28.851490952765644 13.46025436633614 19.219600077210693 38.468654603687526 10 17.108693196361592 26.023677388080085 29.662942556809003 27.204686858749323 11 36.59377444258397 21.788021042607134 22.684862792649675 18.933341722159227 12 26.120021965701472 23.992380647301765 28.07963138807217 21.807965998924598 13 29.41145346226558 19.596243003537914 26.182141110453294 24.81016242374321 14 24.8322157248867 19.839516289339745 25.787080932584544 29.54118705318901 15 24.921707197789782 26.806597473466248 23.892246224070423 24.379449104673544 16 24.848731864627354 25.87968623749562 24.48405803319247 24.78752386468456 17 22.921722368163273 25.600999867008532 25.716545139161983 25.76073262566621 18 24.238436927460036 24.502314831368434 27.65626363190391 23.602984609267622 19 25.1679061784897 24.11870709382151 27.270594965675055 23.44279176201373 20 26.284752317774707 22.957197330518536 26.021437091832794 24.736613259873963 21 28.214879248057073 22.774534397784905 25.401529171827313 23.609057182330716 22 26.427974804483018 23.22933412665267 25.901208858478313 24.441482210386 23 24.09958392274036 23.488126279233754 27.037155170071042 25.375134627954843 24 24.498403817108336 24.8642672433521 26.481332097578836 24.155996841960732 25 24.458038282370744 24.402401122468977 26.646337887761685 24.493222707398598 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 331.0 1 331.0 2 719.0 3 1107.0 4 1107.0 5 1107.0 6 3000.0 7 4893.0 8 4893.0 9 4893.0 10 4527.0 11 4161.0 12 4161.0 13 4161.0 14 3136.5 15 2112.0 16 2112.0 17 2112.0 18 3597.5 19 5083.0 20 5083.0 21 5083.0 22 8113.5 23 11144.0 24 11144.0 25 11144.0 26 16745.5 27 22347.0 28 22347.0 29 22347.0 30 26641.5 31 30936.0 32 30936.0 33 30936.0 34 36152.0 35 41368.0 36 41368.0 37 41368.0 38 46437.5 39 51507.0 40 51507.0 41 51507.0 42 60390.0 43 69273.0 44 69273.0 45 69273.0 46 81852.0 47 94431.0 48 94431.0 49 94431.0 50 96114.0 51 97797.0 52 97797.0 53 97797.0 54 86481.0 55 75165.0 56 75165.0 57 75165.0 58 70024.0 59 64883.0 60 64883.0 61 64883.0 62 57812.5 63 50742.0 64 50742.0 65 50742.0 66 42187.5 67 33633.0 68 33633.0 69 33633.0 70 25442.0 71 17251.0 72 17251.0 73 17251.0 74 13545.5 75 9840.0 76 9840.0 77 9840.0 78 8175.5 79 6511.0 80 6511.0 81 6511.0 82 4480.0 83 2449.0 84 2449.0 85 2449.0 86 1951.0 87 1453.0 88 1453.0 89 1453.0 90 1043.0 91 633.0 92 633.0 93 633.0 94 431.0 95 229.0 96 229.0 97 229.0 98 344.5 99 460.0 100 460.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043158949265368944 2 0.00714552140155115 3 0.002572387704558414 4 0.004573133696992736 5 0.009860819534140587 6 0.01529141579931946 7 0.021579474632684472 8 0.034441413155476545 9 0.05059029152298214 10 0.0603082006290917 11 0.0565925295002851 12 0.06673916989048774 13 0.0653100656101775 14 0.068311184598829 15 0.06302349876168113 16 0.06916864716701514 17 0.06373805090183625 18 0.0788865562731247 19 0.0770287207087214 20 0.08245931697390027 21 0.07674289985265935 22 0.08103021269359004 23 0.08431715253830356 24 0.08160185440571413 25 0.08088730226555901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 699739.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.59627244017794 #Duplication Level Percentage of deduplicated Percentage of total 1 71.26362679808973 28.93037608570416 2 15.10844856855043 12.266933884745793 3 5.600176418589873 6.820388628064035 4 2.6416342325042823 4.289620119601769 5 1.4266806857740484 2.895895890241158 6 0.8462629377816435 2.0613072466925373 7 0.5589565128856065 1.588408561552114 8 0.38654501002023656 1.2553829229738267 9 0.30909333346305057 1.1293233457237892 >10 1.5165281789279177 11.105979415089298 >50 0.15316079950229833 4.389478323960699 >100 0.1676633189771784 14.041649098093641 >500 0.01662484386482567 4.718315754616356 >1k 0.004244640986764 3.654260737825705 >5k 3.537200822303334E-4 0.8526799851150988 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5938 0.8486021216482146 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4141 0.5917920824764662 No Hit TATCAACGCAGAGTACTTTTTTTTT 4054 0.5793588752377672 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2850 0.40729471988841554 No Hit GTACATGGGGTGGTATCAACGCAAA 2744 0.39214621451712717 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2248 0.3212626422137397 No Hit GGTATCAACGCAGAGTACTTTTTTT 1926 0.27524548438775026 No Hit GTATCAACGCAGAGTACATGGGGTG 1784 0.254952203607345 No Hit GTACATGGGTGGTATCAACGCAAAA 1280 0.18292534787970943 No Hit TATCAACGCAGAGTACATGGGGTGG 1169 0.16706229036826586 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1138 0.16263206709930417 No Hit GATTAAGAGGGACGGCCGGGGGCAT 1062 0.15177087456894642 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 1052 0.15034177028863618 No Hit GTACATGGGAGTGGTATCAACGCAA 987 0.14105259246661972 No Hit GAATAGGACCGCGGTTCTATTTTGT 986 0.14090968203858867 No Hit GTATCTGATCGTCTTCGAACCTCCG 972 0.13890893604615434 No Hit ATCAGATACCGTCGTAGTTCCGACC 949 0.13562199620144083 No Hit CTATTGGAGCTGGAATTACCGCGGC 930 0.1329066980688514 No Hit GAACTACGACGGTATCTGATCGTCT 919 0.13133468336051013 No Hit GTCCTATTCCATTATTCCTAGCTGC 895 0.1279048330877656 No Hit GAATAACGCCGCCGCATCGCCAGTC 878 0.1254753558112382 No Hit GCGCAAGACGGACCAGAGCGAAAGC 874 0.1249037140991141 No Hit GGTATCAACGCAGAGTACATGGGGT 859 0.12276005767864877 No Hit CTTTAATATACGCTATTGGAGCTGG 824 0.11775819269756295 No Hit CTCTTAATCATGGCCTCAGTTCCGA 806 0.11518580499300453 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 802 0.11461416328088045 No Hit GTACATGGGGAATAATTGCAATCCC 793 0.11332796942860124 No Hit GCTTTGAACACTCTAATTTTTTCAA 789 0.11275632771647714 No Hit ATATTAAAGTTGCTGCAGTTAAAAA 771 0.11018394001191872 No Hit GAATAATGGAATAGGACCGCGGTTC 770 0.1100410295838877 No Hit TCGTAGTTCCGACCATAAACGATGC 765 0.1093264774437326 No Hit ATCAACGCAGAGTACTTTTTTTTTT 753 0.10761155230736032 No Hit GTCCTGTATTGTTATTTTTCGTCAC 704 0.10060894133384019 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.42910428031023E-4 13 0.0 0.0 0.0 0.0 2.85820856062046E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGTT 60 7.653034E-8 17.419487 17 TATATCT 40 2.764021E-4 16.620558 4 GTCCTAA 50 8.696315E-5 15.201373 1 TTCGCAG 70 7.2314233E-6 13.574596 15 TCGCAGT 70 7.2367693E-6 13.573626 16 GCATCGC 155 0.0 13.486053 14 CGACCAT 120 5.0931703E-11 13.457625 10 TAGACAG 85 2.6982707E-7 13.40914 5 GGACCGT 50 0.0014987044 13.299299 6 CGGTCCA 180 0.0 13.193749 10 CGCATCG 160 0.0 13.062746 13 CCGACCA 125 1.0186341E-10 12.919319 9 AAGACAA 60 4.0937777E-4 12.664188 5 TTAGAGT 75 1.4798918E-5 12.663281 4 GCCGGTC 190 0.0 12.499341 8 CGCCGGT 190 0.0 12.499341 7 GGTCCAA 200 0.0 12.349349 11 GTTTTCG 140 5.2750693E-11 12.217137 15 CGCCCGA 70 1.08760054E-4 12.216263 16 TATTAGC 70 1.09196684E-4 12.211022 2 >>END_MODULE