##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	699739
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.091102539661218	32.0	32.0	32.0	32.0	32.0
2	31.375241339985337	32.0	32.0	32.0	32.0	32.0
3	31.44883306489991	32.0	32.0	32.0	32.0	32.0
4	31.541107470070983	32.0	32.0	32.0	32.0	32.0
5	31.479017176404344	32.0	32.0	32.0	32.0	32.0
6	35.02281136252231	36.0	36.0	36.0	36.0	36.0
7	35.036740841942496	36.0	36.0	36.0	36.0	36.0
8	35.00427159269385	36.0	36.0	36.0	36.0	36.0
9	35.09466243842347	36.0	36.0	36.0	36.0	36.0
10	34.95120752166165	36.0	36.0	36.0	36.0	36.0
11	35.09051231959345	36.0	36.0	36.0	36.0	36.0
12	34.99909966430341	36.0	36.0	36.0	36.0	36.0
13	35.03939468859103	36.0	36.0	36.0	36.0	36.0
14	34.97900359991368	36.0	36.0	36.0	36.0	36.0
15	34.97340294023915	36.0	36.0	36.0	36.0	36.0
16	34.95856026318384	36.0	36.0	36.0	36.0	36.0
17	34.90532327053373	36.0	36.0	36.0	32.0	36.0
18	34.90341112900667	36.0	36.0	36.0	32.0	36.0
19	34.90304670741519	36.0	36.0	36.0	32.0	36.0
20	34.88317215418892	36.0	36.0	36.0	32.0	36.0
21	34.878650468246015	36.0	36.0	36.0	32.0	36.0
22	34.875396397799754	36.0	36.0	36.0	32.0	36.0
23	34.83315493348234	36.0	36.0	36.0	32.0	36.0
24	34.792252539875584	36.0	36.0	36.0	32.0	36.0
25	34.762914458105094	36.0	36.0	36.0	32.0	36.0
26	34.70025538093489	36.0	36.0	36.0	32.0	36.0
27	34.70346086183563	36.0	36.0	36.0	32.0	36.0
28	34.682806017672306	36.0	36.0	36.0	32.0	36.0
29	34.635528389871084	36.0	36.0	36.0	32.0	36.0
30	34.60630320733873	36.0	36.0	36.0	32.0	36.0
31	34.58631146756148	36.0	36.0	36.0	32.0	36.0
32	34.5156737011943	36.0	36.0	36.0	32.0	36.0
33	34.451384016040265	36.0	36.0	36.0	32.0	36.0
34	34.40749622359194	36.0	36.0	36.0	32.0	36.0
35	34.325069776016484	36.0	36.0	36.0	32.0	36.0
36	34.2937423810878	36.0	36.0	36.0	32.0	36.0
37	34.262746538352154	36.0	36.0	36.0	32.0	36.0
38	34.185126168471385	36.0	36.0	36.0	32.0	36.0
39	34.17940403493302	36.0	36.0	36.0	32.0	36.0
40	34.12412771047491	36.0	36.0	36.0	32.0	36.0
41	34.0689156957094	36.0	36.0	36.0	32.0	36.0
42	33.9556020173236	36.0	36.0	36.0	32.0	36.0
43	33.92638255120838	36.0	36.0	36.0	32.0	36.0
44	33.807059489323876	36.0	36.0	36.0	32.0	36.0
45	33.72939195900186	36.0	36.0	36.0	27.0	36.0
46	33.65867559189927	36.0	36.0	36.0	27.0	36.0
47	33.54620365593457	36.0	36.0	36.0	21.0	36.0
48	33.44448001326209	36.0	36.0	36.0	21.0	36.0
49	33.379907365460554	36.0	36.0	36.0	21.0	36.0
50	32.78280616058273	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	9.0
20	14.0
21	40.0
22	173.0
23	387.0
24	983.0
25	2150.0
26	4262.0
27	7448.0
28	11873.0
29	16645.0
30	22537.0
31	29536.0
32	40820.0
33	61253.0
34	117486.0
35	384120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55643230681498	18.365564780420733	12.39768522422425	24.68031768854003
2	15.86367449484592	21.49650511549339	37.00218796490681	25.63763242475387
3	17.720422731462627	26.82844219597419	28.8886507034806	26.562484369082583
4	11.867424854124181	15.886351911212609	35.90467302808619	36.341550206577026
5	13.717829076269867	37.81066940673594	32.139269070324794	16.3322324466694
6	32.29927815736484	36.336652199326316	17.294211968210913	14.06985767509793
7	28.920395919615626	31.529801154146263	21.337129039726296	18.21267388651181
8	29.0376840507675	30.536242799100812	19.9221423988087	20.503930751322994
9	28.707705235718816	13.260505450658252	19.375132193468357	38.65665712015458
10	16.940938732169933	26.05244015200127	29.968373560696897	27.0382475551319
11	36.64123337415808	21.59419440677167	22.811648343167953	18.9529238759023
12	25.899265153528894	24.109452395039284	28.277155163789875	21.714127287641947
13	29.426669486777868	19.47491625413013	26.381378119748017	24.717036139343982
14	24.594741753711027	19.798810699417928	26.089013189203403	29.517434357667643
15	24.872838904506626	26.594140656821537	24.160393767069742	24.37262667160209
16	24.86076832163125	25.648497355419853	24.689845984111084	24.800888338837815
17	22.93569459469888	25.388895002279423	25.96954007136947	25.705870331652232
18	24.1549670161318	23.806121165696776	28.450158345433135	23.58875347273829
19	25.177101747937446	23.717557546456607	27.897973387220095	23.207367318385856
20	26.117707249591277	22.6928441583683	26.592886460036357	24.59656213200407
21	27.844667797564522	22.54140472376129	26.063575133013885	23.55035234566031
22	26.081296026089728	22.89182109329336	26.752403396123412	24.274479484493504
23	23.920123818656545	22.92731656217443	27.831758685098457	25.320800934070565
24	24.47965792617745	24.273293203683696	27.297308112248693	23.949740757890165
25	24.376001291943503	23.732173251910403	27.424216223323295	24.467609232822795
26	24.057818569513483	24.783266114625285	27.80796505961075	23.35095025625048
27	24.54594826354152	24.1251965127912	26.90481634986423	24.42403887380306
28	23.312801017564418	24.242186048505808	28.10550085035229	24.339512083577482
29	23.482779466555765	24.2302245050499	27.666185043381823	24.62081098501251
30	23.29622272084149	24.91203498592274	27.799945691786597	23.991796601449174
31	24.225728536923494	24.71908988266232	26.24819562949306	24.806985950921124
32	23.837596503714376	24.768834123656237	26.185127066201336	25.208442306428054
33	23.71948509287565	24.0783536920876	27.008899541088383	25.19326167394837
34	24.542746976656442	24.19332249093211	26.991719568628685	24.272210963782758
35	25.447968741514394	24.173448008757862	26.82538865879488	23.553194590932865
36	23.912142442666486	25.523883843710966	26.071278502396815	24.492695211225733
37	24.96366388125485	25.420562884709103	25.932055388106907	23.683717845929138
38	23.813130916122724	25.346552766490827	26.544391197652633	24.29592511973382
39	24.581715384956187	25.09307520476545	25.852254456533704	24.47295495374466
40	24.866053755711203	25.630644213908116	26.291479032357184	23.2118229980235
41	23.390799915965303	26.08276845726307	26.660435522665786	23.86599610410584
42	25.186395187810035	26.512062630498068	25.64843516648731	22.653107015204586
43	24.180829231763017	25.252382340667566	26.044828591517895	24.521959836051515
44	23.928978218710387	26.04308239332656	25.23934265980115	24.788596728161902
45	24.05903394580504	26.297744997020295	25.221477334926323	24.421743722248344
46	23.903900213081887	25.91996067060056	25.106076477960816	25.070062638356738
47	24.33280645742166	25.727074466716783	25.578158182821177	24.36196089304038
48	25.553753260209366	26.51241559183965	23.948551216549358	23.98527993140162
49	24.27308091360431	26.927661082639975	24.255645716800956	24.543612286954758
50	24.36178316276584	27.11372929001905	24.162704384449274	24.36178316276584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	78.0
1	71.5
2	65.0
3	147.0
4	229.0
5	286.5
6	344.0
7	358.0
8	372.0
9	545.5
10	719.0
11	1061.5
12	1404.0
13	2523.0
14	3642.0
15	5108.0
16	6574.0
17	7168.5
18	7763.0
19	7735.0
20	7707.0
21	7578.5
22	7450.0
23	7542.5
24	7635.0
25	6521.5
26	5408.0
27	5741.0
28	6074.0
29	6803.5
30	7533.0
31	8715.5
32	9898.0
33	11599.5
34	13301.0
35	15157.0
36	17013.0
37	20215.5
38	23418.0
39	25451.0
40	27484.0
41	30234.5
42	32985.0
43	32666.5
44	32348.0
45	37070.5
46	41793.0
47	45468.5
48	49144.0
49	51737.0
50	54330.0
51	51364.5
52	48399.0
53	47521.0
54	46643.0
55	48260.0
56	49877.0
57	49284.5
58	48692.0
59	43758.5
60	38825.0
61	34093.5
62	29362.0
63	25584.5
64	21807.0
65	18483.0
66	15159.0
67	13113.0
68	11067.0
69	10344.5
70	9622.0
71	7359.5
72	5097.0
73	4466.0
74	3835.0
75	2989.0
76	2143.0
77	1957.5
78	1772.0
79	1441.5
80	1111.0
81	908.0
82	705.0
83	608.0
84	511.0
85	353.5
86	196.0
87	135.0
88	74.0
89	60.5
90	47.0
91	37.5
92	28.0
93	24.0
94	20.0
95	19.0
96	18.0
97	12.5
98	7.0
99	9.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008574625681861379
2	2.85820856062046E-4
3	5.71641712124092E-4
4	0.0
5	0.0
6	2.85820856062046E-4
7	1.42910428031023E-4
8	0.0
9	0.0010003729962171609
10	0.001143283424248184
11	0.0
12	1.42910428031023E-4
13	4.2873128409306896E-4
14	0.0
15	0.005144775409116828
16	8.574625681861379E-4
17	0.0
18	4.2873128409306896E-4
19	0.0
20	4.2873128409306896E-4
21	0.0
22	0.0
23	8.574625681861379E-4
24	0.0010003729962171609
25	0.002572387704558414
26	0.004716044125023759
27	0.005573506693209897
28	0.004144402412899667
29	0.0031440294166825058
30	0.004144402412899667
31	0.007002610973520127
32	0.003572760700775575
33	0.00571641712124092
34	0.002429477276527391
35	0.003572760700775575
36	0.008717536109892402
37	0.0031440294166825058
38	0.008860446537923426
39	0.005144775409116828
40	0.002572387704558414
41	0.0040014919848686435
42	0.002286566848496368
43	0.0010003729962171609
44	0.00142910428031023
45	8.574625681861379E-4
46	0.001143283424248184
47	0.002429477276527391
48	0.0020007459924343217
49	7.14552140155115E-4
50	0.002286566848496368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	699739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.56943491760073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17844314296887	33.347003021748655
2	14.34221184333303	13.071329783384138
3	5.265175310750511	7.197931909590108
4	2.416909033378472	4.405487155932063
5	1.2877122118347855	2.934015891490247
6	0.788873690440854	2.15691169768512
7	0.5176012623990724	1.6510757928113757
8	0.3617936312677558	1.3189385066926749
9	0.25442712756984925	1.0434690387959709
>10	1.3073797581102191	10.71074317851095
>50	0.12347338811777939	4.015472878526286
>100	0.1405259553113142	12.76593265403533
>500	0.013894701199319466	4.376237464399931
>1k	0.0015789433181044843	1.0054510263971035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1856	0.2652417544255787	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1707	0.24394810064895628	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1337	0.19107124227747774	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1060	0.15148505371288437	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1027	0.1467690095878606	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	999	0.14276751760299197	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	988	0.14119550289465072	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	949	0.13562199620144083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.1336212502090065	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	935	0.1336212502090065	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	907	0.12961975822413785	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	885	0.12647572880745536	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	848	0.1211880429703075	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	816	0.11661490927331475	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	806	0.11518580499300453	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	789	0.11275632771647714	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	788	0.11261341728844614	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	784	0.11204177557632203	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	770	0.1100410295838877	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	767	0.10961229829979464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	759	0.10846901487554644	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	750	0.10718282102326726	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	737	0.10532498545886394	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	730	0.10432461246264678	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	722	0.1031813290383986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.42910428031023E-4
3	0.0	0.0	0.0	0.0	1.42910428031023E-4
4	0.0	0.0	0.0	0.0	1.42910428031023E-4
5	0.0	0.0	0.0	0.0	2.85820856062046E-4
6	0.0	0.0	0.0	0.0	2.85820856062046E-4
7	0.0	0.0	0.0	0.0	2.85820856062046E-4
8	0.0	0.0	0.0	0.0	2.85820856062046E-4
9	0.0	0.0	0.0	0.0	2.85820856062046E-4
10	0.0	0.0	0.0	0.0	2.85820856062046E-4
11	0.0	0.0	0.0	0.0	2.85820856062046E-4
12	0.0	0.0	0.0	0.0	5.71641712124092E-4
13	0.0	0.0	0.0	0.0	5.71641712124092E-4
14	0.0	0.0	0.0	0.0	5.71641712124092E-4
15	0.0	0.0	0.0	0.0	8.574625681861379E-4
16	0.0	0.0	0.0	0.0	8.574625681861379E-4
17	0.0	0.0	0.0	1.42910428031023E-4	8.574625681861379E-4
18	0.0	0.0	0.0	4.2873128409306896E-4	8.574625681861379E-4
19	0.0	0.0	0.0	4.2873128409306896E-4	8.574625681861379E-4
20	0.0	0.0	0.0	5.71641712124092E-4	0.001143283424248184
21	0.0	0.0	0.0	8.574625681861379E-4	0.001286193852279207
22	0.0	0.0	0.0	0.00142910428031023	0.0015720147083412529
23	0.0	0.0	0.0	0.002715298132589437	0.0017149251363722758
24	0.0	0.0	0.0	0.005430596265178874	0.0017149251363722758
25	0.0	0.0	0.0	0.0065738796894270575	0.0017149251363722758
26	0.0	0.0	0.0	0.008431715253830357	0.0020007459924343217
27	0.0	0.0	0.0	0.01143283424248184	0.0020007459924343217
28	0.0	0.0	0.0	0.01672052007962969	0.0020007459924343217
29	0.0	0.0	0.0	0.03015410031454585	0.0020007459924343217
30	0.0	0.0	0.0	0.052448127087385436	0.002143656420465345
31	0.0	0.0	0.0	0.09389215121638211	0.002143656420465345
32	0.0	0.0	0.0	0.13819438390599922	0.002143656420465345
33	0.0	0.0	0.0	0.17620855776225136	0.002143656420465345
34	0.0	0.0	0.0	0.21722385060715496	0.002143656420465345
35	0.0	0.0	0.0	0.2678141421301371	0.002143656420465345
36	0.0	0.0	0.0	0.33398167030850073	0.002143656420465345
37	0.0	0.0	0.0	0.422586135687735	0.002143656420465345
38	0.0	0.0	0.0	0.5399155971012048	0.002143656420465345
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAG	40	7.0303533E-4	27.499195	1
GTATAAT	50	0.0025797992	21.999357	1
GTCCTAA	125	9.949872E-10	21.119383	1
ATTCGCT	55	0.004482514	19.999416	10
TATACAG	80	8.983695E-5	19.249435	5
AGCGGGC	185	1.8189894E-12	19.02919	29
CGCAATA	260	0.0	18.616173	36
GTCTATT	60	0.0074072536	18.334108	9
CGGTCCG	180	1.4551915E-11	18.334108	39
GTATTAT	60	0.007410308	18.332798	1
GTCCATA	60	0.007410308	18.332798	1
GCAATAC	285	0.0	17.755135	37
ATACGAA	275	0.0	17.600744	40
TCCCCGC	125	4.8467336E-7	17.599485	1
TCTATTG	75	0.0012903864	17.599485	10
AACGCAG	4410	0.0	17.409924	6
TCGTTTA	360	0.0	17.113058	30
TAGCGGC	285	0.0	16.984386	30
TCTAGCG	285	0.0	16.984386	28
GTATCAA	4455	0.0	16.937777	1
>>END_MODULE