FastQCFastQC Report
Thu 2 Feb 2017
SRR4064242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303037
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35550.2728241792059627No Hit
GTCCTAAAGTGTGTATTTCTCATTT32760.2514126613442289No Hit
GTCCTACAGTGGACATTTCTAAATT28700.22025468194686718No Hit
CTGTAGGACGTGGAATATGGCAAGA27680.21242681520171722No Hit
CTTTAGGACGTGAAATATGGCGAGG25990.19945711441808636No Hit
TATCAACGCAGAGTACTTTTTTTTT23740.18218976130378492No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20910.16047126827557467No Hit
GTCCTACAGTGTGCATTTCTCATTT20770.15939685519290703No Hit
ATTTAGAAATGTCCACTGTAGGACG14690.1127366298884836No Hit
CTGTAGGACCTGGAATATGGCGAGA14260.10943664684886154No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13860.10636689518409685No Hit
CTGAAGGACCTGGAATATGGCGAGA13440.1031436559360939No Hit
GATATACACTGTTCTACAAATCCCG13120.10068785460428216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCG402.7582177E-416.626959
TACTCCG508.717768E-515.1988635
TTAATGC508.72348E-515.1976973
GGATAGG400.005275734714.2511231
ACGCCCC801.287226E-714.25057617
TACCGTC1600.014.2505767
ATACCGT1800.013.7217236
CGAACGA1051.36788E-913.57197816
GCGTTAT500.00149842913.3010491
GTCGTAG1650.013.24295911
TAATACC957.39401E-812.9985284
ATTTAGA5500.012.7828261
GCACGCC751.47753235E-512.66717915
CACGAAT905.400052E-712.66717814
TAGGGCC604.0951432E-412.6652334
TCCAACG2800.012.55407818
CGGACCA2050.012.5136629
TAACGAA1155.316906E-912.39180613
AACGAAC1155.316906E-912.39180614
CGTCGTA1850.012.32529510
Produced by FastQC (version 0.11.5)