##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064242_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303037
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249662902895313	32.0	32.0	32.0	32.0	32.0
2	30.884476803037828	32.0	32.0	32.0	32.0	32.0
3	30.893706778855858	32.0	32.0	32.0	32.0	32.0
4	30.9283619728373	32.0	32.0	32.0	32.0	32.0
5	30.861682362051116	32.0	32.0	32.0	32.0	32.0
6	34.50773155328667	36.0	36.0	36.0	32.0	36.0
7	34.44448622717544	36.0	36.0	36.0	32.0	36.0
8	34.414164755106725	36.0	36.0	36.0	32.0	36.0
9	34.525302811815784	36.0	36.0	36.0	32.0	36.0
10	34.25310102475985	36.0	36.0	36.0	32.0	36.0
11	34.50910142996707	36.0	36.0	36.0	32.0	36.0
12	34.33807865778178	36.0	36.0	36.0	32.0	36.0
13	34.40926312913601	36.0	36.0	36.0	32.0	36.0
14	34.315374774469184	36.0	36.0	36.0	32.0	36.0
15	34.26451973351486	36.0	36.0	36.0	32.0	36.0
16	34.25904866861033	36.0	36.0	36.0	32.0	36.0
17	34.21503226692719	36.0	36.0	36.0	32.0	36.0
18	34.19707345225039	36.0	36.0	36.0	32.0	36.0
19	34.19146578339679	36.0	36.0	36.0	32.0	36.0
20	34.161583285816135	36.0	36.0	36.0	32.0	36.0
21	34.13922705187957	36.0	36.0	36.0	32.0	36.0
22	34.118926016682565	36.0	36.0	36.0	32.0	36.0
23	34.08349954759535	36.0	36.0	36.0	32.0	36.0
24	34.06095145417974	36.0	36.0	36.0	32.0	36.0
25	33.684514714470886	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	12.0
5	16.0
6	61.0
7	21.0
8	69.0
9	72.0
10	115.0
11	20.0
12	75.0
13	60.0
14	120.0
15	242.0
16	382.0
17	573.0
18	666.0
19	875.0
20	1342.0
21	1893.0
22	2929.0
23	4600.0
24	6799.0
25	9592.0
26	14087.0
27	18668.0
28	25309.0
29	33846.0
30	45112.0
31	61548.0
32	85258.0
33	121662.0
34	264649.0
35	602358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.700743869734886	17.605735988815464	11.600959069122535	26.092561072327108
2	15.267532949950152	20.151408983077772	40.34748545812192	24.23357260885015
3	19.06991165112324	24.547818292331023	29.10647298268018	27.275797073865554
4	12.100062548686285	15.880152112265296	37.226368684213554	34.79341665483486
5	13.399237092354808	37.69132173365774	34.73263694345734	14.176804230530122
6	32.68662639107745	36.67094700339331	17.60968394843026	13.032742657098975
7	28.77186141337722	30.869651322738328	21.54024710766132	18.818240156223133
8	26.263573866013612	35.78188155182539	19.977967058064863	17.976577524096133
9	27.511428672339157	13.98298252645448	18.948255025621418	39.557333775584944
10	15.444413303796642	27.47979590504747	33.13310323380697	23.942687557348915
11	36.45009505849399	20.615023849085812	24.409608432717324	18.52527265970288
12	25.009330091734384	23.26264477000364	30.886750348246633	20.841274790015344
13	30.05877646054206	20.358126340977194	25.507632339186404	24.075464859294343
14	22.12355129335369	21.10772879044322	26.051454619734667	30.717265296468426
15	23.57009628549467	30.46289065349585	22.54773000745619	23.419283053553286
16	23.46119200456461	26.03108755768154	27.11487467679522	23.39284576095863
17	22.455270068957045	27.02471088722682	27.087294395896365	23.43272464791977
18	22.897823230294602	25.443775166715692	29.50239887809153	22.15600272489818
19	25.371535300365487	24.517989059186835	26.075707109378182	24.0347685310695
20	25.364232205423125	25.56360949699475	26.35942902521708	22.71272927236505
21	25.2685517062355	24.661905555508625	25.150509532078363	24.919033206177517
22	25.065165667485367	26.472160397399207	25.66467444814625	22.79799948696917
23	23.053734007370167	26.22683766434256	26.758555644136816	23.96087268415046
24	24.6181412109501	25.354594132953217	27.028951224682267	22.998313431414413
25	23.805372874808285	25.78162740705298	27.09608397161726	23.31691574652147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	284.0
1	284.0
2	460.5
3	637.0
4	637.0
5	637.0
6	1758.5
7	2880.0
8	2880.0
9	2880.0
10	2916.5
11	2953.0
12	2953.0
13	2953.0
14	3285.0
15	3617.0
16	3617.0
17	3617.0
18	6584.0
19	9551.0
20	9551.0
21	9551.0
22	16450.0
23	23349.0
24	23349.0
25	23349.0
26	37193.0
27	51037.0
28	51037.0
29	51037.0
30	68501.0
31	85965.0
32	85965.0
33	85965.0
34	95730.5
35	105496.0
36	105496.0
37	105496.0
38	114356.0
39	123216.0
40	123216.0
41	123216.0
42	133872.0
43	144528.0
44	144528.0
45	144528.0
46	155942.0
47	167356.0
48	167356.0
49	167356.0
50	165962.0
51	164568.0
52	164568.0
53	164568.0
54	148001.0
55	131434.0
56	131434.0
57	131434.0
58	119452.0
59	107470.0
60	107470.0
61	107470.0
62	92981.0
63	78492.0
64	78492.0
65	78492.0
66	63992.0
67	49492.0
68	49492.0
69	49492.0
70	37294.0
71	25096.0
72	25096.0
73	25096.0
74	19076.0
75	13056.0
76	13056.0
77	13056.0
78	10027.0
79	6998.0
80	6998.0
81	6998.0
82	4864.5
83	2731.0
84	2731.0
85	2731.0
86	2040.0
87	1349.0
88	1349.0
89	1349.0
90	972.0
91	595.0
92	595.0
93	595.0
94	415.5
95	236.0
96	236.0
97	236.0
98	443.5
99	651.0
100	651.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040213746808417564
2	0.00583252816305293
3	0.0016116196240014671
4	0.0039906771641941095
5	0.009746461535627922
6	0.01381388249144115
7	0.021871980611448485
8	0.03392075589564993
9	0.04689045667928079
10	0.05387414171662048
11	0.05226252209261901
12	0.06131828950367488
13	0.06254619016958075
14	0.0645415287516778
15	0.05863225679700576
16	0.06477176012653517
17	0.059629926088054286
18	0.07444147787054396
19	0.07213916412197044
20	0.0754391471615925
21	0.0736740399543528
22	0.07451822166216308
23	0.07912284915931013
24	0.07559263474483073
25	0.07505542820349691
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1303037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.31465008981401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81941674708428	38.906468403904135
2	13.99769430371926	14.36573570519073
3	4.465972922107724	6.875095134256261
4	1.8495626047879763	3.796386315355998
5	0.9779933940088877	2.5092694401857836
6	0.5975869099997455	1.839897791093407
7	0.4179254965501684	1.501199043335856
8	0.2891755689581694	1.1871154508489075
9	0.2143131483672152	0.9897663796299037
>10	1.1853247339262851	10.980739009133123
>50	0.09361443441557125	3.3647275606249085
>100	0.08062922240117858	8.232618321621429
>500	0.007793148764218107	2.6402037807858636
>1k	0.0029973649093146563	2.8107776640337456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3555	0.2728241792059627	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3276	0.2514126613442289	No Hit
GTCCTACAGTGGACATTTCTAAATT	2870	0.22025468194686718	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2768	0.21242681520171722	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2599	0.19945711441808636	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2374	0.18218976130378492	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2091	0.16047126827557467	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2077	0.15939685519290703	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1469	0.1127366298884836	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1426	0.10943664684886154	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1386	0.10636689518409685	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1344	0.1031436559360939	No Hit
GATATACACTGTTCTACAAATCCCG	1312	0.10068785460428216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.674379161911749E-5	0.0
11	0.0	0.0	0.0	3.0697516647646995E-4	0.0
12	0.0	0.0	0.0	3.0697516647646995E-4	7.674379161911749E-5
13	0.0	0.0	0.0	3.837189580955875E-4	7.674379161911749E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTCG	40	2.7582177E-4	16.62695	9
TACTCCG	50	8.717768E-5	15.198863	5
TTAATGC	50	8.72348E-5	15.197697	3
GGATAGG	40	0.0052757347	14.251123	1
ACGCCCC	80	1.287226E-7	14.250576	17
TACCGTC	160	0.0	14.250576	7
ATACCGT	180	0.0	13.721723	6
CGAACGA	105	1.36788E-9	13.571978	16
GCGTTAT	50	0.001498429	13.301049	1
GTCGTAG	165	0.0	13.242959	11
TAATACC	95	7.39401E-8	12.998528	4
ATTTAGA	550	0.0	12.782826	1
GCACGCC	75	1.47753235E-5	12.667179	15
CACGAAT	90	5.400052E-7	12.667178	14
TAGGGCC	60	4.0951432E-4	12.665233	4
TCCAACG	280	0.0	12.554078	18
CGGACCA	205	0.0	12.513662	9
TAACGAA	115	5.316906E-9	12.391806	13
AACGAAC	115	5.316906E-9	12.391806	14
CGTCGTA	185	0.0	12.325295	10
>>END_MODULE