FastQCFastQC Report
Thu 2 Feb 2017
SRR4064242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303037
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA13590.10429481281038068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG407.036015E-427.49765
ACCGTCG2250.021.5092358
TTAGGAC13350.021.0917933
GTATCAA26500.020.7560941
TAGGACC11000.020.3982224
TGTAGGA22250.020.2678972
TAGGACG22200.020.0162724
ATACCGT2700.019.553856
GTAGGAC21550.019.4971393
GACGTGA12450.019.4360567
TACCGTC2500.019.3583137
AGGACGT22450.019.2054525
CTGTAGG22900.019.0230961
GGACGTG22150.018.8696866
TTTAGGA15150.018.5858382
CCGTCGT2500.018.478399
CGTCGTA2500.018.4783910
AGGACCT16800.018.4626755
CTTTAGG14450.018.4233671
CCTTACG600.00741613318.3317342
Produced by FastQC (version 0.11.5)