##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303037
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319713101009413	32.0	32.0	32.0	32.0	32.0
2	31.441055012252146	32.0	32.0	32.0	32.0	32.0
3	31.502311906722525	32.0	32.0	32.0	32.0	32.0
4	31.579859205839895	32.0	32.0	32.0	32.0	32.0
5	31.543616182809853	32.0	32.0	32.0	32.0	32.0
6	35.12730950847904	36.0	36.0	36.0	36.0	36.0
7	35.14997808964749	36.0	36.0	36.0	36.0	36.0
8	35.10213754482797	36.0	36.0	36.0	36.0	36.0
9	35.19140592323932	36.0	36.0	36.0	36.0	36.0
10	35.07535933361831	36.0	36.0	36.0	36.0	36.0
11	35.196711221553954	36.0	36.0	36.0	36.0	36.0
12	35.113566230275886	36.0	36.0	36.0	36.0	36.0
13	35.14729128950291	36.0	36.0	36.0	36.0	36.0
14	35.09532269613219	36.0	36.0	36.0	36.0	36.0
15	35.07033491758101	36.0	36.0	36.0	36.0	36.0
16	35.08225936792278	36.0	36.0	36.0	36.0	36.0
17	35.061701241023854	36.0	36.0	36.0	36.0	36.0
18	35.05871590752987	36.0	36.0	36.0	36.0	36.0
19	35.03490154155254	36.0	36.0	36.0	36.0	36.0
20	35.03253015839151	36.0	36.0	36.0	36.0	36.0
21	35.02373992449946	36.0	36.0	36.0	36.0	36.0
22	34.9979478710121	36.0	36.0	36.0	36.0	36.0
23	34.960932805438375	36.0	36.0	36.0	36.0	36.0
24	34.93330197070382	36.0	36.0	36.0	36.0	36.0
25	34.90211559610356	36.0	36.0	36.0	32.0	36.0
26	34.857426918805835	36.0	36.0	36.0	32.0	36.0
27	34.832423791496325	36.0	36.0	36.0	32.0	36.0
28	34.795304354365996	36.0	36.0	36.0	32.0	36.0
29	34.763833260298824	36.0	36.0	36.0	32.0	36.0
30	34.73598984526149	36.0	36.0	36.0	32.0	36.0
31	34.73709035123331	36.0	36.0	36.0	32.0	36.0
32	34.68134749819077	36.0	36.0	36.0	32.0	36.0
33	34.68633200745643	36.0	36.0	36.0	32.0	36.0
34	34.66354907803846	36.0	36.0	36.0	32.0	36.0
35	34.62836358445693	36.0	36.0	36.0	32.0	36.0
36	34.5862534985576	36.0	36.0	36.0	32.0	36.0
37	34.54908034077313	36.0	36.0	36.0	32.0	36.0
38	34.491923099651046	36.0	36.0	36.0	32.0	36.0
39	34.446001149622	36.0	36.0	36.0	32.0	36.0
40	34.46172671996267	36.0	36.0	36.0	32.0	36.0
41	34.44079945542605	36.0	36.0	36.0	32.0	36.0
42	34.401663191451966	36.0	36.0	36.0	32.0	36.0
43	34.382340639598105	36.0	36.0	36.0	32.0	36.0
44	34.30310113987554	36.0	36.0	36.0	32.0	36.0
45	34.262779184320934	36.0	36.0	36.0	32.0	36.0
46	34.19923762717406	36.0	36.0	36.0	32.0	36.0
47	34.17761506388537	36.0	36.0	36.0	32.0	36.0
48	34.125899725027	36.0	36.0	36.0	32.0	36.0
49	34.08968663207568	36.0	36.0	36.0	32.0	36.0
50	33.54093628960651	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	3.0
20	18.0
21	43.0
22	138.0
23	381.0
24	958.0
25	2310.0
26	4742.0
27	8714.0
28	14978.0
29	23397.0
30	33326.0
31	47297.0
32	67541.0
33	108129.0
34	222500.0
35	768558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87684606564096	18.55866808503453	12.082989870336004	26.481495978988505
2	14.643123555288145	20.79510187041261	39.907661232145436	24.654113342153813
3	18.503925828660275	24.93958345004785	29.402772139240867	27.153718582051006
4	11.862057639192134	16.52247787284628	37.333015102410755	34.282449385550834
5	13.036314394756252	38.13629236928806	34.470932137767385	14.356461098188309
6	32.758803287092604	36.94886403752468	17.304049926479394	12.988282748903329
7	28.70626751678388	31.053785160195112	21.551131357844298	18.688815965176712
8	26.253667393942	36.001126598860964	19.811716781641657	17.933489225555377
9	27.503973464700053	13.865185388701162	18.907552384117192	39.72328876248159
10	15.437356727174773	27.72626074330345	32.942677055857125	23.89370547366466
11	36.495845087933105	20.54486598988823	24.530097403295073	18.42919151888359
12	24.997122101188456	23.291410661579054	30.855679897838428	20.855787339394062
13	30.108255540172586	20.350675503519494	25.589473134882752	23.951595821425173
14	22.27407552368125	21.07556589040202	25.938827429808104	30.711531156108624
15	23.54986262260357	30.50499623938971	22.449615496784293	23.49552564122243
16	23.521881775100248	26.100880643118895	26.966098117841174	23.41113946393968
17	22.44573442330066	27.16456030378286	27.006774947123485	23.382930325792994
18	23.020333298798572	25.462401240181578	29.493451825929462	22.023813635090388
19	25.44803059930808	24.54828569586719	26.110637004656816	23.893046700167915
20	25.502232870898695	25.502232870898695	26.35793008761879	22.637604170583817
21	25.376869574693583	24.5700620934018	25.246328385149464	24.806739946755158
22	25.193241015597422	26.28615019078521	25.704585291580585	22.81602350203678
23	23.11614517821114	26.164110962654053	26.73976791071293	23.979975948421874
24	24.673222678254035	25.33683082788705	27.002336097152174	22.98761039670675
25	23.966032624336634	25.702697206095092	27.05509913546659	23.27617103410168
26	22.428749592279207	26.960148890040102	27.765920297780077	22.845181219900613
27	23.40963093491168	26.281765937459706	26.737110965119637	23.57149216250898
28	22.811344966047372	26.614668393973258	26.368312315041077	24.205674324938293
29	23.53250867609205	25.508785070828534	25.99136151330078	24.967344739778635
30	22.943107313370636	26.0647568643673	26.80551068037762	24.18662514188444
31	24.56268669087828	25.076441568249425	26.22237435492833	24.13849738594396
32	23.512581773082957	26.634770943272272	26.053341683320593	23.799305600324175
33	22.827347444053288	26.334228971872957	27.390575917837634	23.447847666236115
34	23.81015239412342	26.237161387963038	27.314979320845765	22.637706897067776
35	23.629163468917884	26.408211818879508	27.096930161166537	22.86569455103607
36	22.751975734746146	26.58785985158101	27.017045389133592	23.64311902453925
37	23.4928399681657	26.379953292294612	25.736897322096674	24.390309417443017
38	23.69470699070183	26.966449082655064	25.8146736815445	23.524170245098603
39	24.783957439231795	25.399083948843575	25.859718922104953	23.957239689819676
40	25.243091743246005	25.71515403612877	26.514766624251923	22.52698759637331
41	24.250438413040726	25.237088400185726	27.49028200415197	23.022191182621576
42	24.375581824586554	27.10809485400376	26.43787897741619	22.078444343993496
43	22.95022332734724	26.591610259243907	27.11124925173827	23.34691716167058
44	22.561280717103344	26.968964405765067	26.300824239075375	24.168930638056207
45	22.913256327785465	27.166519700726692	26.16661409660459	23.753609874883253
46	23.369431331814305	25.60814165784746	26.16576632717511	24.856660683163128
47	24.514531253957156	25.75150477468095	26.292862959020553	23.44110101234134
48	23.562809523480617	26.3169689281211	25.537006210998985	24.5832153373993
49	23.958143871806975	26.105591944959265	25.91189031760467	24.02437386562909
50	23.260379397305016	27.640301639122832	25.226973770043916	23.872345193528236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	88.0
2	56.0
3	141.5
4	227.0
5	275.5
6	324.0
7	286.5
8	249.0
9	359.5
10	470.0
11	728.5
12	987.0
13	1632.5
14	2278.0
15	3054.5
16	3831.0
17	4004.5
18	4178.0
19	4245.5
20	4313.0
21	4789.5
22	5266.0
23	6129.5
24	6993.0
25	8370.0
26	9747.0
27	13403.5
28	17060.0
29	21710.0
30	26360.0
31	31651.5
32	36943.0
33	45513.5
34	54084.0
35	63041.5
36	71999.0
37	73754.5
38	75510.0
39	73208.5
40	70907.0
41	69851.0
42	68795.0
43	67691.5
44	66588.0
45	71473.5
46	76359.0
47	79569.5
48	82780.0
49	85700.0
50	88620.0
51	86252.5
52	83885.0
53	82398.0
54	80911.0
55	80018.0
56	79125.0
57	76223.5
58	73322.0
59	66753.0
60	60184.0
61	52901.0
62	45618.0
63	39742.5
64	33867.0
65	28591.5
66	23316.0
67	19936.0
68	16556.0
69	14531.5
70	12507.0
71	9715.5
72	6924.0
73	5752.0
74	4580.0
75	3632.0
76	2684.0
77	2267.5
78	1851.0
79	1521.5
80	1192.0
81	922.5
82	653.0
83	542.0
84	431.0
85	303.5
86	176.0
87	128.0
88	80.0
89	61.5
90	43.0
91	36.0
92	29.0
93	26.0
94	23.0
95	20.5
96	18.0
97	14.0
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010053436702104391
2	6.906941245720575E-4
3	0.0
4	0.0
5	0.0
6	7.67437916191175E-5
7	7.67437916191175E-5
8	0.0
9	0.001381388249144115
10	0.00122790066590588
11	7.67437916191175E-5
12	2.3023137485735247E-4
13	6.906941245720575E-4
14	1.53487583238235E-4
15	0.0043743961222896975
16	9.209254994294099E-4
17	7.67437916191175E-5
18	1.53487583238235E-4
19	7.67437916191175E-5
20	4.6046274971470495E-4
21	0.0
22	7.67437916191175E-5
23	3.0697516647647E-4
24	6.906941245720575E-4
25	0.0016883634156205846
26	0.004758115080385285
27	0.005295321621719107
28	0.0034534706228602874
29	0.0023790575401926423
30	0.0030697516647646994
31	0.006216247121148518
32	0.0031464954563838175
33	0.004297652330670579
34	0.002609288915049995
35	0.002839520289907347
36	0.007981354328388219
37	0.0030697516647646994
38	0.007060428828958808
39	0.004067420955813227
40	0.0023790575401926423
41	0.0032232392480029343
42	0.0018418509988588198
43	0.0013046444575249975
44	0.0013046444575249975
45	0.0010744130826676448
46	7.674379161911748E-4
47	0.001381388249144115
48	0.0019953385820970547
49	1.53487583238235E-4
50	0.001765107207239702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1303037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.48260693434546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30001916642819	44.43406617761162
2	13.540315200636977	15.566652328907166
3	4.182823434286626	7.213187860466013
4	1.6927589914418637	3.8921679895852686
5	0.8714503715918445	2.504661958650164
6	0.5473722870098696	1.8878631612565238
7	0.3529954119836008	1.4203767561676466
8	0.2424657708449215	1.115005168040936
9	0.18315816708603203	0.9475568032879393
>10	0.9607695948577442	9.940820099175365
>50	0.06513859027510444	2.6751734522529667
>100	0.05562716128886594	6.246804881632689
>500	0.004434029601462525	1.713872089724474
>1k	6.718226668882613E-4	0.4417912732412794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1359	0.10429481281038068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.674379161911749E-5
2	0.0	0.0	0.0	0.0	7.674379161911749E-5
3	0.0	0.0	0.0	0.0	7.674379161911749E-5
4	0.0	0.0	0.0	0.0	7.674379161911749E-5
5	0.0	0.0	0.0	0.0	1.5348758323823497E-4
6	0.0	0.0	0.0	0.0	1.5348758323823497E-4
7	0.0	0.0	0.0	0.0	1.5348758323823497E-4
8	0.0	0.0	0.0	0.0	1.5348758323823497E-4
9	0.0	0.0	0.0	1.5348758323823497E-4	1.5348758323823497E-4
10	0.0	0.0	0.0	2.3023137485735247E-4	2.3023137485735247E-4
11	0.0	0.0	0.0	2.3023137485735247E-4	2.3023137485735247E-4
12	0.0	0.0	0.0	2.3023137485735247E-4	2.3023137485735247E-4
13	0.0	0.0	0.0	2.3023137485735247E-4	2.3023137485735247E-4
14	0.0	0.0	0.0	3.0697516647646995E-4	2.3023137485735247E-4
15	0.0	0.0	0.0	3.837189580955875E-4	2.3023137485735247E-4
16	0.0	0.0	0.0	3.837189580955875E-4	2.3023137485735247E-4
17	0.0	0.0	0.0	3.837189580955875E-4	2.3023137485735247E-4
18	0.0	0.0	0.0	3.837189580955875E-4	2.3023137485735247E-4
19	0.0	0.0	0.0	5.372065413338224E-4	2.3023137485735247E-4
20	0.0	0.0	0.0	6.139503329529399E-4	4.6046274971470495E-4
21	0.0	0.0	0.0	8.441817078102924E-4	4.6046274971470495E-4
22	0.0	0.0	0.0	9.976692910485274E-4	5.372065413338224E-4
23	0.0	0.0	0.0	0.0013046444575249975	6.139503329529399E-4
24	0.0	0.0	0.0	0.0022255699569544074	6.139503329529399E-4
25	0.0	0.0	0.0	0.002609288915049995	6.139503329529399E-4
26	0.0	0.0	0.0	0.0032232392480029347	6.139503329529399E-4
27	0.0	0.0	0.0	0.004144164747432345	6.139503329529399E-4
28	0.0	0.0	0.0	0.007444147787054396	6.139503329529399E-4
29	0.0	0.0	0.0	0.013660394908202913	6.139503329529399E-4
30	0.0	0.0	0.0	0.026092889150499947	6.139503329529399E-4
31	0.0	0.0	0.0	0.052492753467476366	6.139503329529399E-4
32	0.0	0.0	0.0	0.0781251798682616	6.139503329529399E-4
33	0.0	0.0	0.0	0.10122506114561597	6.139503329529399E-4
34	0.0	0.0	0.0	0.1263202810050674	6.906941245720575E-4
35	0.0	0.0	0.0	0.15939685519290703	6.906941245720575E-4
36	0.0	0.0	0.0	0.203140816415804	7.67437916191175E-4
37	0.0	0.0	0.0	0.27274743541434354	7.67437916191175E-4
38	0.0	0.0	0.0	0.3620004650673772	7.67437916191175E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	7.036015E-4	27.4976	5
ACCGTCG	225	0.0	21.509235	8
TTAGGAC	1335	0.0	21.091793	3
GTATCAA	2650	0.0	20.756094	1
TAGGACC	1100	0.0	20.398222	4
TGTAGGA	2225	0.0	20.267897	2
TAGGACG	2220	0.0	20.016272	4
ATACCGT	270	0.0	19.55385	6
GTAGGAC	2155	0.0	19.497139	3
GACGTGA	1245	0.0	19.436056	7
TACCGTC	250	0.0	19.358313	7
AGGACGT	2245	0.0	19.205452	5
CTGTAGG	2290	0.0	19.023096	1
GGACGTG	2215	0.0	18.869686	6
TTTAGGA	1515	0.0	18.585838	2
CCGTCGT	250	0.0	18.47839	9
CGTCGTA	250	0.0	18.47839	10
AGGACCT	1680	0.0	18.462675	5
CTTTAGG	1445	0.0	18.423367	1
CCTTACG	60	0.007416133	18.331734	2
>>END_MODULE